Department of Biochemistry and Molecular Biology, Faculty of Veterinary Medicine, Cairo University, Giza, 12211, Egypt.
Department of Surgery, Anesthesiology, and Radiology, Faculty of Veterinary Medicine, Cairo University, Giza, 12211, Egypt.
Sci Rep. 2023 May 17;13(1):7966. doi: 10.1038/s41598-023-35207-3.
One of the most orthopedic problems seen in the equine is osteoarthritis (OA). The present study tracks some biochemical, epigenetic, and transcriptomic factors along different stages of monoiodoacetate (MIA) induced OA in donkeys in serum and synovial fluid. The aim of the study was the detection of sensitive noninvasive early biomarkers. OA was induced by a single intra-articular injection of 25 mg of MIA into the left radiocarpal joint of nine donkeys. Serum and synovial samples were taken at zero-day and different intervals for assessment of total GAGs and CS levels as well as miR-146b, miR-27b, TRAF-6, and COL10A1 gene expression. The results showed that the total GAGs and CS levels increased in different stages of OA. The level of expression of both miR-146b and miR-27b were upregulated as OA progressed and then downregulated at late stages. TRAF-6 gene was upregulated at the late stage while synovial fluid COL10A1 was over-expressed at the early stage of OA and then decreased at the late stages (P < 0.05). In conclusion, both miR-146b and miR-27b together with COL10A1 could be used as promising noninvasive biomarkers for the very early diagnosis of OA.
在马科动物中，最常见的骨科问题之一是骨关节炎（OA）。本研究跟踪了一些生物化学、表观遗传和转录组学因素，这些因素在驴的单碘乙酸（MIA）诱导的 OA 的不同阶段存在于血清和滑液中。本研究的目的是检测敏感的非侵入性早期生物标志物。通过向 9 只驴的左桡腕关节单次关节内注射 25mg MIA 来诱导 OA。在第 0 天和不同时间点采集血清和滑膜样本，以评估总 GAG 和 CS 水平以及 miR-146b、miR-27b、TRAF-6 和 COL10A1 基因表达。结果表明，OA 不同阶段总 GAG 和 CS 水平升高。随着 OA 的进展，miR-146b 和 miR-27b 的表达水平上调，然后在晚期下调。TRAF-6 基因在晚期上调，而滑膜 COL10A1 在 OA 的早期过表达，然后在晚期下降（P < 0.05）。总之，miR-146b 和 miR-27b 与 COL10A1 一起可以作为 OA 早期诊断的有前途的非侵入性生物标志物。
