Orthopedic Institute of Henan Province, Luoyang, Henan 471002, China.
Department of Orthopedics, The Third Affiliated Hospital, Xinxiang Medical University, Xinxiang, Henan 453003, China.
Biomed Res Int. 2019 Jul 29;2019:7165406. doi: 10.1155/2019/7165406. eCollection 2019.
Early detection and treatment are critical in the management of osteoarthritis (OA). OA is closely associated with angiogenesis and the inhibition of angiogenesis presents a novel therapeutic approach to reduce inflammation and pain in OA. Recent reports suggest that circulating microRNAs (miRNAs) have great potential as biomarkers for the diagnosis and prognosis in OA. In this study, we aimed to explore the clinical significance of miR-210 in synovial fluid samples from 10 healthy volunteers and 20 early-stage OA and 20 late-stage OA patients. miR-210 expression was assessed by real-time RT-PCR. VEGF protein levels were examined by ELISA. The results show that miR-210 is significantly upregulated in early-stage OA and late-stage OA patients compared with healthy individuals. Higher levels of VEGF are also found in OA compared with the control. Moreover, miR-210 levels are positively correlated with VEGF levels, suggesting that miR-210 might contribute to OA development through promoting VEGF expression and angiogenesis. In conclusion, upregulation of miR-210 in synovial fluid may occur in the early stage of OA and can be a useful biomarker for early diagnosis of OA.
早期发现和治疗对骨关节炎（OA）的管理至关重要。OA 与血管生成密切相关，抑制血管生成为减轻 OA 炎症和疼痛提供了一种新的治疗方法。最近的报告表明，循环 microRNAs（miRNAs）作为 OA 诊断和预后的生物标志物具有很大的潜力。在这项研究中，我们旨在探讨 miR-210 在 10 名健康志愿者和 20 名早期 OA 和 20 名晚期 OA 患者的滑液样本中的临床意义。通过实时 RT-PCR 评估 miR-210 的表达。通过 ELISA 检查 VEGF 蛋白水平。结果表明，与健康个体相比，早期和晚期 OA 患者的 miR-210 表达显著上调。OA 中的 VEGF 水平也高于对照组。此外，miR-210 水平与 VEGF 水平呈正相关，表明 miR-210 可能通过促进 VEGF 表达和血管生成促进 OA 的发展。总之，滑液中 miR-210 的上调可能发生在 OA 的早期阶段，可作为 OA 早期诊断的有用生物标志物。
