Bende S, Bertók L
Circ Shock. 1986;19(2):239-44.
Circulating endotoxin is an important factor in the pathogenesis and clinical symptoms of endotoxin shock. The effect of extracorporeal activated charcoal hemoperfusion was investigated in experimental endotoxin shock of dogs produced by i.v. injection of Escherichia coli 089 endotoxin (1 mg/kg body weight). The endotoxin was labeled with 99mTc. The aorta and vein cava caudalis of anesthetized dogs were cannulated through the arteria and vein femorales. The cannulae were contacted to the hemoperfusion charcoal cartridge. The efficiency of hemoperfusion was tested from the blood samples, and the endotoxin content of blood was measured biologically (in lead acetate-treated rats) and isotopically (99mTc radioactivity) at 15, 30, 60, 90, and 120 min after injection. It was demonstrated that extracorporeal activated charcoal hemoperfusion can eliminate the majority of circulating endotoxin from the blood within 30 min.
循环内毒素是内毒素休克发病机制和临床症状的一个重要因素。通过静脉注射大肠杆菌089内毒素（1毫克/千克体重）建立犬实验性内毒素休克模型，研究体外活性炭血液灌流的效果。内毒素用99mTc标记。通过股动脉和股静脉对麻醉犬的主动脉和尾腔静脉进行插管。插管与血液灌流炭柱相连。从血样检测血液灌流效率，并在注射后15、30、60、90和120分钟通过生物学方法（在醋酸铅处理的大鼠中）和同位素方法（99mTc放射性）测量血液内毒素含量。结果表明，体外活性炭血液灌流可在30分钟内清除血液中大部分循环内毒素。
