用于筛选在非允许温度下裂解的酵母突变体的简单且灵敏的方法。
Simple and sensitive procedure for screening yeast mutants that lyse at nonpermissive temperatures.
作者信息
Cabib E, Duran A
出版信息
J Bacteriol. 1975 Dec;124(3):1604-6. doi: 10.1128/jb.124.3.1604-1606.1975.
Abstract
After mutagenesis, surviving yeast cells are grown on plates at 25 C and later exposed to 37 C. The plates are then overlaid with a soft agar containing p-nitrophenylphosphate at pH 9.7. Lysed cells liberate alkaline phosphatase which gives rise to a yellow color on and around colonies.
摘要
诱变处理后，存活的酵母细胞在25℃的平板上生长，随后置于37℃环境中。接着，在平板上覆盖一层pH值为9.7、含有对硝基苯磷酸酯的软琼脂。裂解的细胞释放出碱性磷酸酶，使菌落及其周围呈现黄色。
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