一个控制酿酒酵母中非特异性碱性磷酸酶合成的基因。
A gene controlling the synthesis of non specific alkaline phosphatase in Saccharomyces cerevisiae.
作者信息
Toh-E A, Nakamura H, Oshima Y
出版信息
Biochim Biophys Acta. 1976 Mar 25;428(1):182-92. doi: 10.1016/0304-4165(76)90119-7.
Recessive mutants defective in the formation of non-specific alkaline phosphatase (EC 3.1.3.1) could be selected by staining colonies on a plate with p-nitrophenylphosphate after treatment with chloroform vapour. Since no complementation was observed among the nine mutants so far tested, all the mutations might occur in the same locus, phoH. The non-specific alkaline phosphatase was repressible, although a significant basal level of the enzyme activity was observed in the repressed condition.
通过用氯仿蒸汽处理后，用对硝基苯磷酸酯对平板上的菌落进行染色，可以筛选出在非特异性碱性磷酸酶（EC 3.1.3.1）形成过程中存在缺陷的隐性突变体。由于在目前测试的九个突变体中未观察到互补作用，所有突变可能都发生在同一基因座phoH中。尽管在阻遏条件下观察到该酶活性有显著的基础水平，但非特异性碱性磷酸酶是可阻遏的。
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