Xu Jianghao, Li Xingling, Chen Wenlong, Cheng Steven Yan, Cheng Jie, Chen Cong
Department of General Surgery, the First Affiliated Hospital of Nanjing Medical University, Nanjing, China.
Department of Medical Genetics, Jiangsu Key Laboratory of Xenotransplantation, Nanjing Medical University, Nanjing, China.
J Gastrointest Oncol. 2023 Apr 29;14(2):705-718. doi: 10.21037/jgo-23-188.
Colorectal cancer (CRC) is the third most common cancer worldwide and one of the leading causes of cancer-related death. Peptidomics, considered a novel branch of proteomics, has an increasing range of applications in the screening, diagnosis, prognosis, and even monitoring of cancer. However, there is little information for peptidomics analysis in CRC.
In this study, a comparative peptidomic profiling was analyzed in 3 CRC tissue samples and 3 adjacent intestinal epithelial tissue samples by liquid chromatography-tandem mass spectrometry (LC-MS/MS).
Among the total 133 nonredundant peptides identified, 59 were significantly differentially expressed in the CRC samples and benign colonic epithelium conditions [fold change (FC) >2, P<0.05]. Totals of 25 up-regulated and 34 down-regulated peptides were detected, respectively. Gene Ontology (GO) analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis were applied to predict the possible functions of these relevant precursor proteins. To clearly identify the potential interaction network of peptide precursors, the Search Tool for the Retrieval of Interacting Genes/Proteins (STRING) was used to determine protein interactions and a possible central role in CRC.
Our results for the first time revealed the differentially expressed peptides between the serous CRC tissue and the adjacent intestinal epithelial tissue samples, and these prominently variable peptides might have an important potential role in occurrence and development of CRC.
结直肠癌（CRC）是全球第三大常见癌症，也是癌症相关死亡的主要原因之一。肽组学作为蛋白质组学的一个新分支，在癌症的筛查、诊断、预后甚至监测中的应用范围越来越广。然而，关于CRC的肽组学分析的信息很少。
在本研究中，通过液相色谱-串联质谱（LC-MS/MS）对3个CRC组织样本和3个相邻肠上皮组织样本进行了比较肽组学分析。
在总共鉴定出的133种非冗余肽中，59种在CRC样本和良性结肠上皮条件下有显著差异表达[倍数变化（FC）>2，P<0.05]。分别检测到25种上调肽和34种下调肽。应用基因本体（GO）分析和京都基因与基因组百科全书（KEGG）分析来预测这些相关前体蛋白的可能功能。为了明确鉴定肽前体的潜在相互作用网络，使用了检索相互作用基因/蛋白质的搜索工具（STRING）来确定蛋白质相互作用以及在CRC中可能的核心作用。
我们的结果首次揭示了浆液性CRC组织与相邻肠上皮组织样本之间差异表达的肽，这些显著变化的肽可能在CRC的发生和发展中具有重要的潜在作用。
