Fractions 14 and 36K of Metabolite Extract subsp. Hygroscopicus Have Antimalarial Activities Against in vitro.

PURPOSE

The study was conducted to investigate the effectivity and the cytotoxicity of fractions 14 and 36K of metabolite extract of subsp. Hygroscopicus as an antimalarial compounds against in vitro.

METHODS

Fractions 14 and 36K of metabolite extract of subsp. Hygroscopicus produced by the fractionation process utilizing the Flash Column Chromatography (FCC) BUCHI Reveleris PREP. culture was used to assess the antimalarial activity of fractions 14 and 36K. Parasite densities and the ability of parasite growth were determined under microscopic. The cytotoxicity of the fractions was assessed using MTT assays on the MCF-7 cell line.

RESULTS

subsp. Hygroscopicus fractions 14 and 36K have antimalarial activity against , with fraction 14 having the more potent activity. The percentage of -infected erythrocytes was decreased as well as the increase of fraction concentration. Fraction 14 has the highest inhibition of parasite growth at a concentration of 156,25 μg/mL, with an inhibition percentage of 67.73% (R = 0.953, p = 0.000). IC of fractions 14 and 36K were found at 10.63 μg/mL and 135,91 μg/mL, respectively. The fractions caused morphological damage in almost all asexual stages of the parasite. Both fractions were not toxic against MCF-7, indicating that the fractions have a safe active metabolite.

CONCLUSION

Fractions 14 and 36K of metabolite extract subsp. Hygroscopicus contains non-toxic compounds that could damage the morphology and inhibit the growth of in vitro.