Guangdong Provincial Key Laboratory of Food Quality and Safety, South China Agricultural University, Guangzhou 510642, China.
Future Technology Institute, South China Normal University, 510631, China.
J Hazard Mater. 2023 Aug 5;455:131634. doi: 10.1016/j.jhazmat.2023.131634. Epub 2023 May 13.
Bongkrekic acid (BA) is a mitochondrial toxin that causes high mortality but is often mistakenly categorized as other food poisonings. The immunoassay of BA is still challenging since the specific antibody is unavailable. In this work, a monoclonal antibody specific to BA was first generated and a dual-modular immunosensor for on-site and laboratory detection was established. The antibody showed good affinity (Kd=0.33 μM) and sensitivity (IC =17.9 ng/mL in ELISA) with negligible cross-reactivity with common mycotoxins. In dual-modular conditions, fluorescence assay (FA) was conducted based on the inner filter effect of carbon dots (CDs) and oxidized 3,3',5,5'-tetramethylbenzidine (TMB), while the colorimetric assay (CA) was conducted using TMB-mediated rapid surface etching of gold nanostars (Au NSs). The proposed immunosensor showed good sensitivity and reproducibility to BA in food samples, with a limit of detection lower than 10 ng/mL and recovery ranging from 80.0% to 103.6%, which was in good consistence with that of standard LC-MS/MS. Overall, the proposed immunosensor is an ideal tool for screening BA contaminants in food with good sensitivity and high effectivity.
邦克酸（BA）是一种线粒体毒素，可导致高死亡率，但常被错误归类为其他食物中毒。由于缺乏特异性抗体，BA 的免疫检测仍然具有挑战性。本研究首次制备了针对 BA 的单克隆抗体，并建立了用于现场和实验室检测的双模块免疫传感器。该抗体具有良好的亲和力（Kd=0.33 μM）和灵敏度（ELISA 中 IC50 为 17.9 ng/mL），与常见真菌毒素的交叉反应性可忽略不计。在双模块条件下，荧光分析（FA）基于碳点（CDs）和氧化 3,3',5,5'-四甲基联苯胺（TMB）的内滤效应进行，而比色分析（CA）则使用 TMB 介导的金纳米星（Au NSs）快速表面蚀刻进行。该免疫传感器对食品样品中的 BA 具有良好的灵敏度和重现性，检测限低于 10 ng/mL，回收率在 80.0%至 103.6%之间，与标准 LC-MS/MS 的结果吻合较好。总体而言，该免疫传感器是一种用于筛选食品中 BA 污染物的理想工具，具有灵敏度高、效果好的特点。
