Department of Neurosurgery, Affiliated Hospital of Guizhou Medical University, Guiyang, China.
Department of Neurosurgery, Affiliated Hospital of Guizhou Medical University, Guiyang, China.
Eur J Pharmacol. 2023 Jul 15;951:175800. doi: 10.1016/j.ejphar.2023.175800. Epub 2023 May 17.
Every year, there is a large number of people take aspirin and atorvastatin to prevent ischemic stroke, but the effect of these drugs on gut microbiota remains unknown. We aimed to examine the effects of long-term regular oral aspirin with atorvastatin to prevent ischemic stroke on human gut microbiota.
A cross-sectional study of 20 participants with the drugs over one year and the other 20 gender- and age-matching participants without medication were recruited from the Affiliated Hospital of Guizhou Medical University. The medication habits and dietary information were obtained using a questionnaire. Fecal samples collected from all participants were subjected to 16S rRNA sequencing of the microbiome. The datasets were analyzed using bioinformatics approaches.
The Alpha diversity showed that compared with controls, medication participants had lower ACE and Chao1 index, while no difference in the Shannon index and Simpson index. The Beta diversity analysis revealed significant shifts in the taxonomic compositions of the two groups. Linear discriminant analysis effect size (LEfSe) analysis combined with receiver operating characteristic (ROC) curves revealed the marker bacteria associated with taking medication were g_Parabacteroides（AUC = 0.855）, g_Bifidobacterium（AUC = 0.815）, s_Bifidobacterium_longum_subsp（AUC = 0.8075）, and with no taking medication was g_Prevotella_9（AUC = 0.76）.
Our findings indicated that long-term regular oral aspirin and atorvastatin modulate the human gut microbiota. Taking these drugs may affect the preventive effect of ischemic stroke by changing the abundance of specific gut microbiota.
每年都有大量的人服用阿司匹林和阿托伐他汀来预防缺血性中风，但这些药物对肠道微生物群的影响尚不清楚。我们旨在研究长期定期口服阿司匹林联合阿托伐他汀预防缺血性中风对人类肠道微生物群的影响。
从贵州医科大学附属医院招募了 20 名服用上述药物超过一年的患者和 20 名性别和年龄匹配的未服用药物的患者进行横断面研究。使用问卷获得药物使用习惯和饮食信息。收集所有参与者的粪便样本进行 16S rRNA 微生物组测序。使用生物信息学方法分析数据集。
Alpha 多样性分析显示，与对照组相比，用药组的 ACE 和 Chao1 指数较低，而 Shannon 指数和 Simpson 指数无差异。Beta 多样性分析显示两组的分类组成有明显变化。线性判别分析效应量（LEfSe）分析结合受试者工作特征（ROC）曲线显示与用药相关的标记细菌为 g_Parabacteroides（AUC=0.855）、g_Bifidobacterium（AUC=0.815）、s_Bifidobacterium_longum_subsp（AUC=0.8075），与未用药相关的标记细菌为 g_Prevotella_9（AUC=0.76）。
我们的研究结果表明，长期定期口服阿司匹林和阿托伐他汀可调节人类肠道微生物群。服用这些药物可能会通过改变特定肠道微生物群的丰度来影响缺血性中风的预防效果。
