Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, China; Key Laboratory of Forestry Genetics & Biotechnology (Nanjing Forestry University), Ministry of Education, Nanjing 210037, China; Jiangsu Province Key Laboratory of Green Biomass-based Fuels and Chemicals, Nanjing 210037, China.
State Key Laboratory of Civilian NBC Protection, Beijing 102205, China.
Bioresour Technol. 2023 Aug;382:129172. doi: 10.1016/j.biortech.2023.129172. Epub 2023 May 16.
Lactic acid (LA) is efficient in xylo-oligosaccharides (XOS) production from poplar. However, the role of LA in XOS production from corncob has not been carefully elucidated, and the co-production of probiotics of Bacillus subtilis from corncob residue has not been reported. In this study, LA pretreatment was combined with enzymatic hydrolysis to produce XOS and monosaccharides from corncob. An XOS yield of 69.9% was obtained from corncob by combining 2% LA pretreatment and xylanase hydrolysis. Yields of 95.6% glucose and 54.0% xylose were obtained from corncob residue via cellulase, and the resulting cellulase hydrolysate was used to culture Bacillus subtilis YS01. The resulting viable count of the strain was 6.4×10 CFU/mL, and the glucose and xylose utilization were 99.0% and 89.8%, respectively. This study demonstrated a green, efficient, and mild process for producing XOS and probiotics from corncob by combining LA pretreatment and enzymatic hydrolysis.
乳酸（LA）在从杨树生产木低聚糖（XOS）方面非常有效。然而，LA 在从玉米芯生产 XOS 方面的作用尚未被仔细阐明，并且利用玉米芯残渣生产枯草芽孢杆菌益生菌也尚未见报道。在本研究中，将 LA 预处理与酶解相结合，从玉米芯生产 XOS 和单糖。通过 2% LA 预处理和木聚糖酶水解，从玉米芯中获得了 69.9%的 XOS 产率。通过纤维素酶从玉米芯残渣中获得了 95.6%的葡萄糖和 54.0%的木糖产率，并且所得纤维素酶水解物用于培养枯草芽孢杆菌 YS01。该菌株的活菌数达到 6.4×10 CFU/mL，葡萄糖和木糖利用率分别为 99.0%和 89.8%。本研究通过 LA 预处理和酶解相结合，展示了一种从玉米芯生产 XOS 和益生菌的绿色、高效和温和的工艺。
