Bank H L, Davis R F, Emerson D
Diabetologia. 1979 Mar;16(3):195-9. doi: 10.1007/BF01219798.
Isolated rat Islets of Langerhans have been frozen to and stored at -196 degrees. After thawing, these islets were capable of secreting near normal levels of insulin in response to graded glucose challenge. Maximal retention of functional viability as measured by the ability of the islets to secrete insulin in response to a glucose challenge was obtained after freezing islets at a cooling rate of approximately 75 degrees per minute in the presence of 1.0 mol/1 dimethyl sulfoxol followed by warming at rates of greater than 3.5 degrees/minute. The critical freezing parameters include the time and temperature of exposure to dimethyl sulfoxide, the rate of cooling, the temperature of the post-thaw dilution from the freezing medium and the presence of serum in the dilution medium.
分离出的大鼠胰岛已被冷冻至-196度并储存于此。解冻后，这些胰岛在接受分级葡萄糖刺激时能够分泌接近正常水平的胰岛素。在1.0摩尔/升二甲基亚砜存在的情况下，以每分钟约75度的冷却速率冷冻胰岛，然后以大于3.5度/分钟的速率升温，通过胰岛对葡萄糖刺激分泌胰岛素的能力来衡量，此时功能性活力的最大保留率得以实现。关键的冷冻参数包括接触二甲基亚砜的时间和温度、冷却速率、从冷冻介质中解冻后稀释的温度以及稀释介质中血清的存在情况。
