Carey H V, Cooke H J
Dig Dis Sci. 1986 Jul;31(7):732-6. doi: 10.1007/BF01296451.
The effects of cholera toxin on intestinal transport parameters were examined in muscle-stripped flat sheets of guinea pig ileum in order to determine whether the effects were partly mediated by stimulation of the mucosal innervation. Cholera toxin evoked an increase in short-circuit current that reflected active ion secretion. Tetrodotoxin completely blocked neurally mediated responses to electrical field stimulation, but it did not prevent the effects of cholera toxin. In the absence of tetrodotoxin, electrical stimulation of submucosal neurons evoked a biphasic increase in short-circuit current that was produced by chloride secretion. The first phase, which was known to be cholinergic, was enhanced by cholera toxin. These results suggest that cholera toxin may mediate intestinal secretion by direct action on the enterocytes as well as by enhancing cholinergically mediated intestinal secretory processes.
为了确定霍乱毒素对肠道转运参数的影响是否部分由刺激黏膜神经支配介导，研究人员在去除肌肉的豚鼠回肠平片上检测了这些影响。霍乱毒素引起短路电流增加，这反映了主动离子分泌。河豚毒素完全阻断了神经介导的对电场刺激的反应，但它并不能阻止霍乱毒素的作用。在没有河豚毒素的情况下，对黏膜下神经元的电刺激会引起由氯离子分泌产生的短路电流双相增加。已知第一阶段是胆碱能性 的，霍乱毒素会增强这一阶段。这些结果表明，霍乱毒素可能通过直接作用于肠上皮细胞以及增强胆碱能介导的肠道分泌过程来介导肠道分泌。
