Liu Xinyi, Zhang Ai, Dong Nianguo, Wang Zhiwen
Department of Cardiovascular Surgery, Institution of Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430022, China.
Department of Rheumatology and Immunology, General Hospital of Central Theater Command, Wuhan, Hubei 430070, China.
Cardiol Res Pract. 2022 Sep 23;2022:3806462. doi: 10.1155/2022/3806462. eCollection 2022.
Sporadic acute Stanford type A aortic dissection (TAAD) is a serious condition that requires urgent treatment to avoid catastrophic consequences. The purpose of the present study was to explore, firstly, whether TLR4-regulated immune signalling molecules were activated in TAAD patients and, secondly, whether TLR4-regulated inflammatory products interleukin-1 (IL-1) and CC chemokine ligand 5 (CCL5) could be a promising biomarker for diagnosis in patients with TAAD. Full-thickness ascending aortic wall specimens from TAAD patients ( = 12) and control donors ( = 12) were examined for the expression of TLR4 and its major signalling molecules, in terms of immunity and inflammation. Blood samples from TAAD ( = 49) and control patients ( = 53) were collected to detect the circulating plasma cytokine levels of IL-1 and CCL5. We demonstrated that expression levels of TLR4 and its downstream signalling cascade molecules were significantly elevated. Furthermore, receiver operating characteristic curve analyses showed that elevated IL-1 levels and decreased plasma CCL5 may have diagnostic value for TAAD. In summary, this current study suggests a more generalized pattern of inflammation in TAAD. In addition, TLR4-mediated inflammatory product, such as IL-1 and CCL5, could be novel and promising biomarkers with important diagnostic and predictive value in the identification of sporadic TAAD diseases.
散发性急性 Stanford A 型主动脉夹层（TAAD）是一种严重疾病，需要紧急治疗以避免灾难性后果。本研究的目的，首先是探讨 TAAD 患者中 Toll 样受体 4（TLR4）调节的免疫信号分子是否被激活，其次是探讨 TLR4 调节的炎性产物白细胞介素 -1（IL -1）和 CC 趋化因子配体 5（CCL5）是否可能成为 TAAD 患者诊断的有前景的生物标志物。对 12 例 TAAD 患者和 12 例对照供体的升主动脉全层壁标本进行了 TLR4 及其主要信号分子在免疫和炎症方面的表达检测。采集了 49 例 TAAD 患者和 53 例对照患者的血样，以检测循环血浆中 IL -1 和 CCL5 的细胞因子水平。我们证明 TLR4 及其下游信号级联分子的表达水平显著升高。此外，受试者工作特征曲线分析表明，IL -1 水平升高和血浆 CCL5 降低可能对 TAAD 具有诊断价值。总之，本研究提示 TAAD 存在更广泛的炎症模式。此外，TLR4 介导的炎性产物，如 IL -1 和 CCL5，可能是散发性 TAAD 疾病识别中具有重要诊断和预测价值的新型且有前景的生物标志物。
