Alfonso-Garcia Alba, Anbunesan Silvia Noble, Bec Julien, Lee Han Sung, Jin Lee-Way, Bloch Orin, Marcu Laura
Biomedical Engineering Department, University of California, Davis, One Shields Ave, Davis, CA 95616, USA.
Pathology and Laboratory Medicine Department, University of California, Davis, 4400 V St, Sacramento, CA 95817, USA.
Biomed Opt Express. 2023 Apr 24;14(5):2196-2208. doi: 10.1364/BOE.481304. eCollection 2023 May 1.
Challenges in identifying a glioblastoma's infiltrative edge during neurosurgical procedures result in rapid recurrence. A label-free fluorescence lifetime imaging (FLIm) device was used to evaluate glioblastoma's infiltrative edge in 15 patients (89 samples). FLIm data were analyzed according to tumor cell density, infiltrating tissue type (gray and white matter), and diagnosis history (new or recurrent). Infiltrations in white matter from new glioblastomas showed decreasing lifetimes and a spectral red shift with increasing tumor cell density. Areas of high versus low tumor cell density were separated through a linear discriminant analysis with a ROC-AUC=0.74. Current results support the feasibility of intraoperative FLIm for real-time brain measurements and encourage refinement to predict glioblastoma infiltrative edge, underscoring the ability of FLIm to optimize neurosurgical outcomes.
在神经外科手术过程中识别胶质母细胞瘤浸润边缘面临的挑战会导致肿瘤快速复发。使用一种无标记荧光寿命成像（FLIm）设备对15例患者（89个样本）的胶质母细胞瘤浸润边缘进行评估。根据肿瘤细胞密度、浸润组织类型（灰质和白质）以及诊断史（新发或复发）对FLIm数据进行分析。新发胶质母细胞瘤在白质中的浸润显示，随着肿瘤细胞密度增加，荧光寿命缩短且光谱发生红移。通过线性判别分析分离出高肿瘤细胞密度区域和低肿瘤细胞密度区域，其ROC-AUC = 0.74。当前结果支持术中FLIm用于实时脑部测量的可行性，并鼓励进一步完善以预测胶质母细胞瘤的浸润边缘，强调了FLIm优化神经外科手术结果的能力。
