转录组分析揭示了与南丹瑶鸡产蛋相关的关键基因和途径。

Transcriptome analysis reveals key genes and pathways associated with egg production in Nandan-Yao domestic chicken.

作者信息

Sun Tiantian, Xiao Cong, Deng Jixian, Yang Zhuliang, Zou Leqin, Du Wenya, Li Shuxia, Huo Xianqiang, Zeng Linghu, Yang Xiurong

机构信息

College of Animal Science and Technology, Guangxi, University, Nanning, Guangxi 530004, China.

出版信息

Comp Biochem Physiol Part D Genomics Proteomics. 2021 Dec;40:100889. doi: 10.1016/j.cbd.2021.100889. Epub 2021 Aug 20.

DOI:10.1016/j.cbd.2021.100889

PMID:34509172

Abstract

BACKGROUND

Egg production is a very important economic trait in chicken breeding, but its molecular mechanism is unclear until now. Nandan-Yao chicken (Gallus gallus domesticus) is a native breed in Guangxi province, China, which is famous for good meet quality, but with low egg production.

METHODS

To explore the molecular regulation related to egg production, high egg production (HEP) and low egg production (LEP) were divided according to the total egg number at 55 weeks, and the concentration of serum sex hormones was tested to evaluate the physiological function of ovary and uterus. RNA sequencing (RNA-Seq) was used to explore the transcriptome from the ovary and uterus of Nandan-Yao chicken.

RESULTS

The levels of serum sex hormone showed that concentrations of estradiol (E2), follicle-stimulating hormone (FSH), and luteotropic hormone (LH) were significantly higher in HEP than those in LEP (P < 0.01), while the concentration of testosterone (T) was significantly lower in HEP (P < 0.01). RNA-Seq analysis identified 901 and 2763 differentially expressed genes (DEGs) in ovary and uterus, respectively. Enrichment analysis showed that DEGs were significantly involved in the regulation of tight junction in the ovary (P < 0.05), while in uterus, DEGs were mainly enriched in the phagosome, ECM-receptor interaction, cell adhesion molecules (CAMs), focal adhesion, cardiac muscle contraction, cytokine-cytokine receptor interaction, and the regulation of MAPK signaling pathway (P < 0.05). Protein network interaction and function analyses revealed that FN1, FGF7, SOX2 identified from the ovary, and UQCRH, COX5A, FN1 from the uterus might be key candidate genes for egg production in Nandan-Yao chicken.

CONCLUSIONS

Our study provided key candidate genes and pathways involved in the egg-laying process of Nandan-Yao chicken and could help to further understand the molecular mechanisms of chicken reproduction.

摘要

背景

产蛋是鸡育种中非常重要的经济性状，但迄今为止其分子机制尚不清楚。南丹瑶鸡（家鸡）是中国广西的一个本土品种，以肉质优良而闻名，但产蛋量较低。

方法

为了探究与产蛋相关的分子调控机制，根据55周龄时的总产蛋数将高产蛋量（HEP）和低产蛋量（LEP）进行分组，并检测血清性激素浓度以评估卵巢和子宫的生理功能。采用RNA测序（RNA-Seq）技术探究南丹瑶鸡卵巢和子宫的转录组。

结果

血清性激素水平显示，高产蛋量组的雌二醇（E2）、促卵泡激素（FSH）和促黄体生成素（LH）浓度显著高于低产蛋量组（P < 0.01），而高产蛋量组的睾酮（T）浓度显著低于低产蛋量组（P < 0.01）。RNA-Seq分析分别在卵巢和子宫中鉴定出901个和2763个差异表达基因（DEG）。富集分析表明，差异表达基因在卵巢中显著参与紧密连接的调控（P < 0.05），而在子宫中，差异表达基因主要富集在吞噬体、细胞外基质-受体相互作用、细胞黏附分子（CAMs）、黏着斑、心肌收缩、细胞因子-细胞因子受体相互作用以及MAPK信号通路的调控（P < 0.05）。蛋白质网络相互作用和功能分析表明，从卵巢中鉴定出的FN1、FGF7、SOX2以及从子宫中鉴定出的UQCRH、COX5A、FN1可能是南丹瑶鸡产蛋的关键候选基因。