School of Life Sciences, B.S. Abdur Rahman Crescent institute of Science and Technology, Vandalur, Chennai 600 048, India.
School of Life Sciences, B.S. Abdur Rahman Crescent institute of Science and Technology, Vandalur, Chennai 600 048, India.
Aquat Toxicol. 2023 Jul;260:106583. doi: 10.1016/j.aquatox.2023.106583. Epub 2023 May 16.
The presence of Vibrio parahaemolyticus, Vibrio alginolyticus, and Vibrio harveyi in aquatic organisms causes vibriosis, leading to their significant mortality. The efficacy of antibiotic treatment is reduced due to increasing antibiotic resistance. As a result, novel therapeutic agents are increasingly needed to treat outbreak of such diseases in aquatic organisms and humans. This study focuses on utilizing the bioactive compounds of Cymbopogon citratus as they are rich in a variety of secondary metabolites which promotes growth, natural immune response and disease resistance against pathogenic bacteria in various ecosystems. In silico studies were performed to evaluate the binding potential of the bioactive compounds against targeted protein beta - lactamase in Vibrio parahaemolyticus and metallo - beta - lactamase in V. alginolyticus via molecular docking. Cymbopogon citratus nanoparticles (CcNps) were synthesized, characterized and toxicity studies were performed by using Vigna radiata, and Artemia nauplii at different concentrations of Cymbopogon citratus nanoparticles. The results revealed that the synthesized nanoparticles were non-ecotoxic and act as potential growth promoters in plants. The antibacterial activity of synthesized Cymbopogon citratus was evaluated using agar well diffusion method. MIC, MBC, and biofilm assays performed by using different concentrations of synthesized nanoparticles. Thus, it was proved that Cymbopogon citratus nanoparticles showed better antibacterial activity against Vibrio species.
副溶血弧菌、溶藻弧菌和哈维弧菌存在于水生生物中会引起弧菌病，导致其大量死亡。由于抗生素耐药性的增加，抗生素治疗的效果降低。因此，越来越需要新型治疗剂来治疗水生生物和人类中此类疾病的爆发。本研究集中于利用香茅草的生物活性化合物，因为它们富含多种次生代谢产物，可在各种生态系统中促进生长、天然免疫反应和对病原菌的抗病性。通过分子对接进行了计算机模拟研究，以评估生物活性化合物对副溶血弧菌中目标蛋白β-内酰胺酶和溶藻弧菌中金属β-内酰胺酶的结合潜力。合成了香茅草纳米粒子（CcNps），并通过不同浓度的香茅草纳米粒子对豇豆和卤虫进行了表征和毒性研究。结果表明，合成的纳米粒子无生态毒性，并在植物中充当潜在的生长促进剂。采用琼脂孔扩散法评价了合成的香茅草的抗菌活性。通过不同浓度的合成纳米粒子进行 MIC、MBC 和生物膜测定。因此，证明了香茅草纳米粒子对弧菌属具有更好的抗菌活性。
