Analytical Investigation of and Exploiting the Potential of Developed Silver Nanoparticle Against the Dominating Species of Pathogenic Bacteria.

Indian biodiversity is a hub for medicinal plants. Extensive research has been carried out to select plants with numerous properties which can be used for human welfare. Present research is about , an economically valuable medicinal plant. In this study was elected as a subject plant over the five selected plants (, , , and ) on the basis of antibacterial effect against dominating pathogenic species of gram positive () and gram negative () bacteria. Further, bioactive agents behind antibacterial potential of was analyzed using analytical method (Phyto-chemical, FTIR, NMR and GC-MS). Due to the broad antimicrobial spectrum, silver nanoparticles have turned into a noteworthy decision for the improvement of new medication. Therefore, this investigation further elaborated in the development of silver nano-particles (CNPs). Antibacterial potential of CNPs examine in a range of C-C (μg/ml) through minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) where, C (μg/ml) concentration of CNPs were recorded as the MIC for all bacterial species and C (μg/ml) and C (μg/ml) noted as the MBC for and , respectively. In agar disk diffusion assay of CNPs, maximum diameter of zone of inhibition was observed for C (μg/ml) concentration (20.12 ± 0.42), (22.34 ± 0.4), (35.23 ± 0.46) and (31.87 ± 0.24). Involvement of bioactive component as a reducing and capping agent can be confirmed through FTIR spectrum of CNPs. Moreover XRD, EDXRF and SEM showed crystalline and cuboidal nature of CNPs with 35 nm sizes. Prominently, cytotoxic analysis was conducted to understand the toxic effect of CNPs. This research highlights the potential of CNPs due to the bioactive components present in extract: Polyphenols (phenol; 1584.56 ± 16.32 mg/L, Flavanoids) and mixture of terpenoids (Citral, Myrcene, Farnesol, β-myrcene and β -Pinene).