Lagente O, Diry M, Courtois Y
FEBS Lett. 1986 Jul 7;202(2):207-10. doi: 10.1016/0014-5793(86)80688-3.
We have purified two growth factors from dogfish brain and retina by using their binding affinity for heparin-Sepharose. These growth factors were eluted at 1 and 2 M NaCl similarly to those purified from bovine brain or retina. Their mitogenic activity was assayed in vitro on the same mammalian cells: bovine lens epithelial cells or human fibroblasts. All these data seem to indicate that these growth factors belong to the families of other well defined mammalian growth factors: EDGF I, brain basic FGF, AGF II, on the one hand and EDGF II, brain acidic FGF, AGF I, ECGF, on the other. Thus, these growth factors have been widely distributed during evolution and retain at least a conservative sequence to stimulate cell proliferation, in mammalian cells.
我们利用狗鱼脑和视网膜中的生长因子与肝素-琼脂糖的结合亲和力，对其进行了纯化。这些生长因子在1M和2M氯化钠浓度下被洗脱，与从牛脑或视网膜中纯化出的生长因子情况类似。我们在相同的哺乳动物细胞（牛晶状体上皮细胞或人成纤维细胞）上对其促有丝分裂活性进行了体外测定。所有这些数据似乎都表明，这些生长因子属于其他已明确的哺乳动物生长因子家族：一方面是EDGF I、脑碱性FGF、AGF II，另一方面是EDGF II、脑酸性FGF、AGF I、ECGF。因此，这些生长因子在进化过程中广泛分布，并且在哺乳动物细胞中至少保留了保守序列以刺激细胞增殖。
