Moenner M, Chevallier B, Badet J, Barritault D
Proc Natl Acad Sci U S A. 1986 Jul;83(14):5024-8. doi: 10.1073/pnas.83.14.5024.
Eye-derived growth factor I (EDGF-I), the retinal form of the basic fibroblast growth factor, has been purified to homogeneity from bovine retina by heparin-Sepharose chromatography. The radioiodinated EDGF-I retained full mitogenic activity and was used to study the interaction of the growth factor with bovine epithelial lens cells. We showed that 125I-labeled EDGF-I bound in a saturable and reversible manner to a specific cellular receptor. Scatchard analysis of the equilibrium binding gave a Kd of 53 X 10(-12) M with approximately equal to 20,000 binding sites per cell. Crosslinking experiments using two homobifunctional reagents induced the formation of a specific major complex with a Mr of approximately equal to 145,000, as determined by NaDodSO4/PAGE, and independent of reducing conditions. These data establish the existence of a receptor for the basic growth factor derived from neural tissues and give an estimation of the size of this receptor at Mr 130,000.
眼源性生长因子I（EDGF-I），即碱性成纤维细胞生长因子的视网膜形式，已通过肝素-琼脂糖凝胶柱色谱法从牛视网膜中纯化至均一状态。放射性碘化的EDGF-I保留了完整的促有丝分裂活性，并用于研究该生长因子与牛晶状体上皮细胞的相互作用。我们发现，125I标记的EDGF-I以可饱和且可逆的方式与一种特异性细胞受体结合。对平衡结合进行Scatchard分析得出解离常数Kd为53×10⁻¹² M，每个细胞约有20,000个结合位点。使用两种同型双功能试剂进行的交联实验诱导形成了一种特异性主要复合物，通过十二烷基硫酸钠/聚丙烯酰胺凝胶电泳（NaDodSO4/PAGE）测定，其分子量约为145,000，且与还原条件无关。这些数据证实了神经组织来源的碱性生长因子存在一种受体，并估计该受体的大小为分子量130,000。
