Department of Emergency, Critical Care, and Disaster Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences.
Department of Emergency, Critical Care, and Disaster Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences.
Surgery. 2023 Aug;174(2):343-349. doi: 10.1016/j.surg.2023.04.029. Epub 2023 May 18.
Lung contusion caused by blunt chest trauma evokes a severe inflammatory reaction in the pulmonary parenchyma that may be associated with acute respiratory distress syndrome. Although hydrogen gas has antioxidant and anti-inflammatory effects and is protective against multiple types of lung injury at safe concentrations, the effects of inhaled hydrogen gas on blunt lung injury have not been previously investigated. Therefore, using a mouse model, we tested the hypothesis that hydrogen inhalation after chest trauma would reduce pulmonary inflammation and acute lung injury associated with lung contusion.
Inbred male C57BL/6 mice were randomly divided into 3 groups: sham with air inhalation, lung contusion with air inhalation, and lung contusion with 1.3% hydrogen inhalation. Experimental lung contusion was induced using a highly reproducible and standardized apparatus. Immediately after induction of lung contusion, mice were placed in a chamber exposed to 1.3% hydrogen gas in the air. Histopathological analysis and real-time polymerase chain reaction in lung tissue and blood gas analysis were performed 6 hours after contusion.
Histopathological examination of the lung tissue after contusion revealed perivascular/intra-alveolar hemorrhage, perivascular/interstitial leukocyte infiltration, and interstitial/intra-alveolar edema. These histological changes and the extent of lung contusion, as determined by computed tomography, were significantly mitigated by hydrogen inhalation. Hydrogen inhalation also significantly reduced inflammatory cytokine and chemokine mRNA levels and improved oxygenation.
Hydrogen inhalation therapy significantly mitigated inflammatory responses associated with lung contusion in mice. Hydrogen inhalation therapy may be a supplemental therapeutic strategy for treating lung contusion.
钝性胸部创伤引起的肺挫裂伤可在肺实质中引起剧烈的炎症反应，可能与急性呼吸窘迫综合征有关。尽管氢气具有抗氧化和抗炎作用，并且在安全浓度下对多种类型的肺损伤具有保护作用，但吸入氢气对钝性肺损伤的影响尚未得到研究。因此，我们使用小鼠模型检验了这样一个假设，即胸部创伤后吸入氢气可减轻与肺挫裂伤相关的肺部炎症和急性肺损伤。
将近交系雄性 C57BL/6 小鼠随机分为 3 组：空气吸入假手术组、空气吸入肺挫裂伤组和 1.3%氢气吸入肺挫裂伤组。使用高度可重复和标准化的装置诱导实验性肺挫裂伤。肺挫裂伤后立即将小鼠置于暴露于空气中 1.3%氢气的室内。挫伤后 6 小时进行肺组织实时聚合酶链反应和血气分析以及组织学分析。
挫伤后肺组织的组织学检查显示血管周围/肺泡内出血、血管周围/间质白细胞浸润和间质/肺泡内水肿。这些组织学变化和 CT 确定的肺挫伤程度，通过吸入氢气明显减轻。氢气吸入还显著降低了炎症细胞因子和趋化因子的 mRNA 水平，并改善了氧合作用。
氢气吸入疗法可显著减轻小鼠肺挫裂伤相关的炎症反应。氢气吸入疗法可能是治疗肺挫裂伤的辅助治疗策略。
