柚皮素和还原氧化石墨烯联合对抗粪肠球菌生物膜的作用。
Combined effect of apigenin and reduced graphene oxide against Enterococcus faecalis biofilms.
机构信息
Department of Conservative Dentistry, School of Dentistry, Jeonbuk National University.
Research Institute of Clinical Medicine of Jeonbuk National University.
出版信息
J Oral Sci. 2023 Jul 1;65(3):163-167. doi: 10.2334/josnusd.22-0459. Epub 2023 May 20.
PURPOSE
Enterococcus faecalis (E. faecalis) is one of the major microorganisms that causes failure of endodontic treatment. This study aimed to investigate the antibacterial activity of apigenin and its synergistic effect with reduced graphene oxide (RGO) in treating E. faecalis biofilms.
METHODS
The antibacterial activities were characterized by viability analysis including colony forming units and confocal laser scanning microscopy (CLSM) analyses. The effect on biofilm biomass was measured using a crystal violet staining method. Live and dead bacteria bio-volumes were determined by CLSM analysis, and the morphology of E. faecalis biofilm after treatment with apigenin and apigenin + RGO was observed by scanning electron microscopy (SEM).
RESULTS
The viability of E. faecalis in biofilms decreased by apigenin treatment in a dose-dependent manner. While apigenin alone did not significantly affect the biofilm biomass, apigenin + RGO reduced the biomass in an apigenin concentration-dependent manner. Likewise, the bio-volume of live bacteria decreased and the bio-volume of dead bacteria increased in apigenin-treated biofilms. According to SEM images, apigenin + RGO-treated samples showed less E. faecalis in biofilms than apigenin-only treated samples.
CONCLUSION
The results suggested that the combined use of apigenin and RGO could be a potential strategy for effective endodontic disinfection.
目的
粪肠球菌（E. faecalis）是导致根管治疗失败的主要微生物之一。本研究旨在探讨芹菜素及其与还原氧化石墨烯（RGO）协同作用治疗粪肠球菌生物膜的抗菌活性。
方法
通过菌落形成单位和共聚焦激光扫描显微镜（CLSM）分析来表征抗菌活性。使用结晶紫染色法测量生物膜生物量的影响。通过 CLSM 分析测定活细菌和死细菌的生物体积，并通过扫描电子显微镜（SEM）观察经芹菜素和芹菜素+RGO 处理后的粪肠球菌生物膜的形态。
结果
粪肠球菌生物膜中的细菌活力随芹菜素处理呈剂量依赖性下降。虽然单独使用芹菜素对生物膜生物量没有显著影响，但芹菜素+RGO 以芹菜素浓度依赖性方式降低了生物膜生物量。同样，活细菌的生物体积减少，死细菌的生物体积增加。根据 SEM 图像，与仅用芹菜素处理的样本相比，用芹菜素+RGO 处理的样本中的粪肠球菌更少。
结论
结果表明，联合使用芹菜素和 RGO 可能是一种有效的牙髓消毒的潜在策略。
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