Department of Biomedical Engineering, Research Center for Nano-biomaterials & Regenerative Medicine, College of Biomedical Engineering, Taiyuan University of Technology, Taiyuan, PR China.
Shanxi-Zheda Institute of Advanced Materials and Chemical Engineering, Taiyuan, PR China.
J Biomater Sci Polym Ed. 2023 Oct;34(15):2076-2090. doi: 10.1080/09205063.2023.2217063. Epub 2023 Jun 1.
The ideal bone repair materials possess a series of properties, such as injectability, good mechanical properties and bone inducibility. In the present study, gelatin methacryloyl (GelMA) and graphene oxide (GO) were selected to prepare conductive hydrogel by changing the concentration of GelMA and GO during the cross-link process. The effects of different contents of GelMA and GO to the hydrogel performance were investigated. The results showed that the mechanical properties of the hydrogel kept 16.37 ± 1.89 KPa after adding 0.1% GO, while the conductivity was improved to 1.36 ± 0.09 μS/cm. The porosity of hydrogel before and after mineralization could reach more than 90%. The mechanical properties of mineralized hydrogel was improved significantly, could reach 26.38 ± 2.29 KPa. Cell experiments indicated that the mineralized hydrogel with electrical stimulation obviously improve the alkaline phosphatase activity of the cells. GelMA/GO conductive hydrogel could be a promising candidate for bone repair and bone tissue engineering.
理想的骨修复材料具有一系列特性，如可注射性、良好的机械性能和骨诱导性。在本研究中，选择明胶甲基丙烯酰（GelMA）和氧化石墨烯（GO）通过在交联过程中改变 GelMA 和 GO 的浓度来制备导电水凝胶。研究了不同含量的 GelMA 和 GO 对水凝胶性能的影响。结果表明，加入 0.1%GO 后水凝胶的力学性能保持在 16.37±1.89kPa，而导电性提高到 1.36±0.09μS/cm。水凝胶在矿化前后的孔隙率均可达到 90%以上。矿化水凝胶的力学性能显著提高，可达到 26.38±2.29kPa。细胞实验表明，电刺激矿化水凝胶明显提高了细胞的碱性磷酸酶活性。GelMA/GO 导电水凝胶有望成为骨修复和骨组织工程的候选材料。
