School of Basic Medical Sciences, People's Hospital of Hebi, Henan University, Kaifeng, China.
Parasitol Res. 2023 Jul;122(7):1567-1572. doi: 10.1007/s00436-023-07858-8. Epub 2023 May 22.
Mites serve as pathogens, allergens, or microbial containers, which can seriously damage the health of humans and animals. The substantial amount of mite species and their similar morphology make it complicated to identify and classify. Our mouse breeder incidentally noticed papular-type erythema with itching and peeling of the skin in several places, and an investigation revealed that this symptom was caused by an uncommon parasite that appeared on the skin and around the nest of the mice. By morphological observation, DNA extraction, PCR amplification, and DNA sequencing, we roughly identified the category of the parasite as a mite. Then, we designed a specific primer cox1, amplified and sequenced the mitochondrial cox1 gene fragment of the mite, calculated the intraspecific and interspecific differences, and reconstructed the phylogenetic tree for sequence alignment. Finally, this species was identified and named this Ornithonyssus bacoti-KF. According to the ivermectin gradient test, we found that 0.1 mg/mL concentration of ivermectin solution was the most effective for mite removal in the bath, with no recurrence after 6 months of treatment. Ornithonyssus bacoti, diagnosed by microscopic exam and confirmed by PCR amplification sequencing, was treated with ivermectin to control the rodent-borne parasite effectively.
螨作为病原体、过敏原或微生物的容器，会严重损害人类和动物的健康。大量的螨种和相似的形态结构使得鉴定和分类变得复杂。我们的老鼠饲养员偶然注意到几只老鼠的皮肤出现丘疹样红斑、瘙痒和脱皮，调查发现这种症状是由一种不常见的寄生虫引起的，这种寄生虫出现在老鼠的皮肤和窝周围。通过形态学观察、DNA 提取、PCR 扩增和 DNA 测序，我们大致将寄生虫鉴定为螨类。然后，我们设计了一个特异性引物 cox1，扩增并测序了螨的线粒体 cox1 基因片段，计算了种内和种间差异，并对序列进行比对构建了系统发育树。最后，确定并将该物种命名为巴氏禽刺螨-KF。根据伊维菌素浓度梯度试验，我们发现 0.1mg/mL 浓度的伊维菌素溶液在沐浴中最有效地去除螨虫，治疗 6 个月后无复发。巴氏禽刺螨通过显微镜检查和 PCR 扩增测序确诊，用伊维菌素治疗有效地控制了啮齿动物传播的寄生虫。
