Tsopmene Ulrich Joël, Iwewe Yves Somo, Eyong Isaac Mboh, Bisso Borel Ndezo, Dzoyem Jean Paul
Department of Biochemistry, University of Dschang, Dschang, CMR.
Department of Biomedical Sciences, University of Ngaoundéré, Ngaoundéré, CMR.
Cureus. 2023 Apr 20;15(4):e37877. doi: 10.7759/cureus.37877. eCollection 2023 Apr.
Background . is one of the most causative agents of urinary tract infections (UTIs). This study aimed to investigate the antibiotic resistance profile and the virulence factors, including the biofilm formation ability of isolates from urine. Methodology The agar disk diffusion method was used to test the susceptibility of isolates to ten antibiotics. The biofilm formation ability was determined using the safranin microplate-based method, and the phospholipase, esterase, and hemolysin activities were assessed by the agar plate method. Results During the study period, a prevalence of 18.12% of urinary tract infections caused by the identified Staphylococci was obtained. All the isolated and were resistant to cefazolin. Multi-drug resistance (MDR) was recorded in 80.01%, 81.49%, and 76.20% of , and isolates, respectively. Most of the isolates were moderate biofilm formers, while 44.44%, 31.75%, and 30.16% were positive for phospholipase, esterase, and hemolysin activities, respectively. No relevant correlations were observed between the ability of biofilm formation and the resistance to antibiotics or the expression of virulence factors investigated. Conclusion This study shows that isolates from patients with clinical manifestations of UTIs expressed a high degree of virulence factors, including the ability of biofilm formation, and exhibited multi-drug resistance to the majority of antimicrobials commonly used for the treatment of Staphylococcal infections.
背景。是尿路感染（UTIs）最常见的病原体之一。本研究旨在调查尿液分离株的抗生素耐药性概况和毒力因子，包括生物膜形成能力。方法采用琼脂纸片扩散法检测分离株对十种抗生素的敏感性。使用基于番红微板的方法测定生物膜形成能力，并通过琼脂平板法评估磷脂酶、酯酶和溶血素活性。结果在研究期间，已鉴定出的葡萄球菌引起的尿路感染患病率为18.12%。所有分离出的和均对头孢唑林耐药。、和分离株的多重耐药（MDR）分别记录为80.01%、81.49%和76.20%。大多数分离株为中度生物膜形成者，而分别有44.44%、31.75%和30.16%的分离株磷脂酶、酯酶和溶血素活性呈阳性。在生物膜形成能力与抗生素耐药性或所研究的毒力因子表达之间未观察到相关相关性。结论本研究表明，有尿路感染临床表现患者的分离株表达了高度的毒力因子，包括生物膜形成能力，并且对大多数常用于治疗葡萄球菌感染的抗菌药物表现出多重耐药性。
