Osei-Owusu Jonathan, Kokro Kwabena Boakye, Ofori Andrew, Apau Joseph, Dofuor Aboagye Kwarteng, Vigbedor Bright Yaw, Aniagyei Albert, Kwakye Ralph, Edusei George, Antwi Boniface Yeboah, Okyere Harry
Department of Physical and Mathematical Sciences, University of Environment and Sustainable Development Somanya, Ghana.
Department of Chemistry, Kwame Nkrumah University of Science and Technology Kumasi, Ghana.
Int J Biochem Mol Biol. 2023 Apr 15;14(2):17-24. eCollection 2023.
In Ghana, and are green leafy vegetables that are customarily eaten together with a starchy staple food. The present study aimed at assessing the ethanolic leaf extract of , and for antioxidant capacity, phytochemical property, nutritional and anti-nutrient content.
Phytochemical constituent and proximate analysis were determined using standard protocols. The DPPH scavenging activity was used to determine the antioxidant activity of the ethanolic leaf extracts from the three vegetables. The antinutrients phytate and oxalate were determined by titrimetric methods of analysis.
Pytochemical screening revealed the presence of tannins and flavonoids in , and . Alkaloids and saponins were present in and but not in . Terpenoids, steroids, carotenoids and coumarins were absent in all the three vegetables. Proximate analysis revealed varying levels of moisture, fat, protein, ash, crude fibre and carbohydrates in the three leafy vegetables. The DPPH scavenging showed 86.71%, 71.72% and 38.86% activity for , and respectively. The antinutrient results revealed an oxalate level of 2.7 ± 0.13% for , 6.43 ± 0.06% for and 12.32 ± 0.13% for . For levels of phytates, our results revealed a 3.084 ± 0.54%, 1.14 ± 0.26% and 1.71 ± 0.27% for , and , respectively.
The current study has shown that , and possess important phytochemicals, nutrients and significant antioxidant activity, suggesting a potential of these vegetables against diverse disease, if eaten by humans.
在加纳,[蔬菜名称1]和[蔬菜名称2]是绿叶蔬菜,通常与含淀粉的主食一起食用。本研究旨在评估[蔬菜名称1]、[蔬菜名称2]和[蔬菜名称3]的乙醇叶提取物的抗氧化能力、植物化学特性、营养成分和抗营养成分。
采用标准方案测定植物化学成分和近似分析。用DPPH清除活性来测定这三种蔬菜乙醇叶提取物的抗氧化活性。通过滴定分析法测定抗营养物质植酸盐和草酸盐。
植物化学筛选显示,[蔬菜名称1]、[蔬菜名称2]和[蔬菜名称3]中存在单宁和黄酮类化合物。[蔬菜名称1]和[蔬菜名称2]中存在生物碱和皂苷,但[蔬菜名称3]中没有。这三种蔬菜中均不存在萜类、甾体、类胡萝卜素和香豆素。近似分析显示,这三种绿叶蔬菜中的水分、脂肪、蛋白质、灰分、粗纤维和碳水化合物含量各不相同。DPPH清除率显示,[蔬菜名称1]、[蔬菜名称2]和[蔬菜名称3]的活性分别为86.71%、71.72%和38.86%。抗营养物质结果显示,[蔬菜名称1]的草酸盐含量为2.7±0.13%,[蔬菜名称2]为6.43±0.06%,[蔬菜名称3]为12.32±0.13%。关于植酸盐含量,我们的结果显示,[蔬菜名称1]、[蔬菜名称2]和[蔬菜名称3]分别为3.084±0.54%、1.14±0.26%和1.71±0.27%。
当前研究表明,[蔬菜名称1]、[蔬菜名称2]和[蔬菜名称3]含有重要的植物化学物质、营养成分和显著的抗氧化活性,表明这些蔬菜如果被人类食用,可能对多种疾病具有预防作用。