Department of Pharmacology and Therapeutics, Mbarara University of Science and Technology, P.O Box, Mbarara 1410, Uganda.
Department of Pharmacology and Therapeutics, Lira University, P.O Box, Lira 1035, Uganda.
ScientificWorldJournal. 2022 Jul 18;2022:1376817. doi: 10.1155/2022/1376817. eCollection 2022.
. Diabetes mellitus is a metabolic disorder that has no known cure with continuous endeavors to find a therapy for the condition. According to some studies, traditional leafy vegetables could prevent and manage diabetes by modifying the carbohydrate and lipid metabolism. In this study, a phytochemical analysis, acute toxicity, as well as antihyperglycemic and antidiabetic activity testing of the methanolic, diethyl ether, and aqueous leaf extracts of L. was performed. . Methanolic, diethyl ether, and aqueous leaf extracts of L. were prepared by serial extraction. Phytochemical analysis was performed following standard methods. 52 mice were separated into 13 groups (A-M) of 4 and received extracts' doses ranging from 1000 mg/kg to 5000 mg/kg for the acute toxicity testing. For the antihyperglycemic and antidiabetic activities testing, 48 rats were divided into 8 groups of 6 and received 500 mg/kg of each extract. 10 mg/kg of glibenclamide and distilled water were used as controls. Data were analyzed using Prism GraphPad version 8.0.2 (263). . Phytochemical analysis revealed the presence of alkaloids, reducing sugars, saponins, and terpenoids. There were no acute toxicity signs observed in this study. L. extracts demonstrated moderate antihyperglycemic and antidiabetic activities. The methanolic extract exhibited the highest degree of antihyperglycemic activity. However, there was no statistically significant difference between the extracts and the negative control ( > 0.05), but with glibenclamide ( < 0.01). . L. is a safe and potential postprandial antidiabetic vegetable that could minimize the rise in blood glucose after a meal. We therefore recommend further investigations into the antidiabetic properties of the vegetable using purified extracts.
. 糖尿病是一种代谢紊乱疾病，目前尚无已知的治愈方法，人们一直在努力寻找该疾病的治疗方法。根据一些研究，传统的绿叶蔬菜可以通过改变碳水化合物和脂质代谢来预防和治疗糖尿病。在这项研究中，对 L. 的甲醇、二乙醚和水提叶片提取物进行了植物化学分析、急性毒性以及降血糖和抗糖尿病活性测试。. L. 的甲醇、二乙醚和水提叶片提取物通过连续提取制备。采用标准方法进行植物化学分析。将 52 只小鼠分为 13 组（A-M），每组 4 只，接受提取物剂量为 1000mg/kg 至 5000mg/kg，进行急性毒性测试。为了进行降血糖和抗糖尿病活性测试，将 48 只大鼠分为 8 组，每组 6 只，分别给予 500mg/kg 的每种提取物。将 10mg/kg 的格列本脲和蒸馏水用作对照。数据使用 Prism GraphPad 版本 8.0.2（263）进行分析。. 植物化学分析表明存在生物碱、还原糖、皂苷和萜类化合物。在这项研究中没有观察到急性毒性迹象。 L. 提取物表现出中等程度的降血糖和抗糖尿病活性。甲醇提取物表现出最高的降血糖活性。然而，与阴性对照相比，提取物之间没有统计学上的显著差异（>0.05），但与格列本脲相比（<0.01）。. L. 是一种安全且有潜力的餐后抗糖尿病蔬菜，可以最大限度地减少餐后血糖升高。因此，我们建议进一步研究该蔬菜的抗糖尿病特性，使用纯化的提取物。
