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Immunocytochemical demonstration of 5-hydroxytryptamine in gastrointestinal endocrine cells.

作者信息

Facer P, Polak J M, Jaffe B M, Pearse A G

出版信息

Histochem J. 1979 Jan;11(1):117-21. doi: 10.1007/BF01041271.

DOI:10.1007/BF01041271
PMID:372152
Abstract

We have been able to demonstrate 5-hydroxytryptamine in the enterochromaffin cells of the mammalian gastrointestinal tract, using a highly specific antiserum. Conventional histochemical techniques for identifying amines as cell markers can thus be replaced by more reliable and sensitive immunocytochemical methods. This has been facilitated by the use of p-benzoquinone as fixative which has been shown to preserve the localization and antigenicity of amines, as well as peptides.

摘要

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1
Immunocytochemical demonstration of 5-hydroxytryptamine in gastrointestinal endocrine cells.
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[Chromatographic detection of melatonin (5-methoxy-N-acetyltryptamine) and its biological precursors in enterochromaffin cells].
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Immunoelectron cytochemical localization of motilin and substance P in rabbit bile duct enterochromaffin (EC) cells.兔胆管肠嗜铬(EC)细胞中胃动素和P物质的免疫电子细胞化学定位
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