Inokuchi H, Azuma T, Kawai K, Takeuchi Y, Sano Y
Histochemistry. 1984;80(5):517-8. doi: 10.1007/BF00495444.
Radioautographic studies of rat duodenal mucosa were carried out after single injections of 3H-thymidine, and the labelling indices of enterochromaffin (EC) cells identified by serotonin immunohistochemistry and by the diazo reaction were compared. The labelling index of EC cells by serotonin immunohistochemistry was 2.3% (mean percentage of 2 rats), while no labelled cells were found in diazo-positive EC cells. This finding indicates that serotonin immunohistochemistry reveals a less mature population of EC cells than conventional techniques such as the diazo reaction.
单次注射3H-胸腺嘧啶核苷后，对大鼠十二指肠黏膜进行放射自显影研究，并比较了通过血清素免疫组织化学和重氮反应鉴定的肠嗜铬（EC）细胞的标记指数。通过血清素免疫组织化学检测的EC细胞标记指数为2.3%（2只大鼠的平均百分比），而在重氮阳性EC细胞中未发现标记细胞。这一发现表明，与重氮反应等传统技术相比，血清素免疫组织化学揭示的EC细胞群体不太成熟。
