Inokuchi H, Kawai K, Takeuchi Y, Sano Y
Histochemistry. 1983;79(1):9-16. doi: 10.1007/BF00494337.
Selective immunohistochemical identification of enterochromaffin (EC) cells in the human duodenum and colon has been carried out, using a highly specific rabbit antiserotonin serum and the unlabeled peroxidase-antiperoxidase complex (PAP) technique. The sensitivity of the reaction, and morphology of the cells, were compared with those of the argentaffin or argyrophil reactions on the same sections at the light microscopic level. All argentaffin cells were argyrophil and PAP positive, but a small number of PAP-positive cells were neither argentaffin nor argyrophil. In addition, a few EC cells stained by the PAP method were shown to be argyrophil but not argentaffin. The PAP method permitted the clear morphological identification of the entire cell, particularly its cytoplasmic processes, whereas both the argentaffin and argyrophil reactions were basically seen in the basal regions of the cells and often failed to demonstrate the cytoplasmic processes. These findings indicate that the immunohistochemical method is more sensitive and advantageous than silver impregnations for the selective demonstration of EC cells.
利用高特异性兔抗血清素血清和未标记的过氧化物酶 - 抗过氧化物酶复合物（PAP）技术，对人十二指肠和结肠中的肠嗜铬（EC）细胞进行了选择性免疫组织化学鉴定。在光学显微镜水平上，将该反应的敏感性以及细胞形态与同一切片上的嗜银或亲银反应进行了比较。所有嗜银细胞均为亲银且PAP阳性，但少数PAP阳性细胞既非嗜银也非亲银。此外，一些用PAP方法染色的EC细胞显示为亲银但非嗜银。PAP方法能够清晰地从形态上鉴定整个细胞，特别是其细胞质突起，而嗜银和亲银反应基本上只能在细胞的基部区域看到，并且常常无法显示细胞质突起。这些发现表明，免疫组织化学方法在选择性显示EC细胞方面比银浸染法更敏感且更具优势。
