Caiye Zhu, Song Shuzhen, Li Minna, Huang Xaioyu, Luo Yan, Fang Suli
College of Animal Science and Technology, Gansu Agricultural University, Lanzhou, Gansu, China.
Institute of Animal and Pasture Science and Green Agriculture, Gansu Academy of Agricultural Sciences, Lanzhou, Gansu, China.
Front Vet Sci. 2023 May 5;10:1125262. doi: 10.3389/fvets.2023.1125262. eCollection 2023.
The study was aimed to analyze the difference of genome-wide DNA differential methylation in Lanzhou Large-tailed sheep, Altay sheep and Tibetan sheep, which the typical breeds with different type tails, as to screen the differentially methylated genes (DMGs) that affect the type of tails.
In this study, three Lanzhou Large-tailed sheep, three Altay sheep and three Tibetan sheep were detected by whole genome bisulfite sequencing (WGBS). The degree of genome-wide DNA methylation, differentially methylated regions (DMRs) and DMGs were analyzed. The candidate genes affecting the tail type of sheep were identified by GO and KEGG pathway enrichment analysis of DMGs.
we identified 68,603 different methylated regions (DMCs) and 75 differentially methylated genes (DMGs) associated with these DMCs. Functional analysis showed that these DMGs were mainly enriched in biological process, cellular component and molecular function, Some of the genes in these pathways are involved in fat metabolism: , , and .
Our results may help to further understand the epigenetic regulation mechanisms of deposition of fat in the tail of sheep and provide new basic data for the study of local sheep.
本研究旨在分析兰州大尾羊、阿勒泰羊和藏羊这三种具有不同尾型的典型品种全基因组DNA差异甲基化情况，以筛选影响尾型的差异甲基化基因（DMG）。
本研究采用全基因组亚硫酸氢盐测序（WGBS）对三只兰州大尾羊、三只阿勒泰羊和三只藏羊进行检测。分析全基因组DNA甲基化程度、差异甲基化区域（DMR）和DMG。通过对DMG进行GO和KEGG通路富集分析，鉴定影响绵羊尾型的候选基因。
我们鉴定出68,603个不同的甲基化区域（DMC）以及与这些DMC相关的75个差异甲基化基因（DMG）。功能分析表明，这些DMG主要富集在生物过程、细胞成分和分子功能方面，这些通路中的一些基因参与脂肪代谢： 、 、 和 。
我们的研究结果可能有助于进一步了解绵羊尾部脂肪沉积的表观遗传调控机制，并为地方绵羊品种的研究提供新的基础数据。
