Ganguli Sumon, Howlader Sabbir, Ullah A K M Atique, Bhuiyan Farhana Rumzum, Akhi Aklima A, Hasan Abid, Dey Kamol, Islam Saiful, Ali Ferdousi, Chakraborty Ashok Kumar, Bhattacharjee Samiran, Dey Benu Kumar
Department of Applied Chemistry and Chemical Engineering, University of Chittagong, Chattogram, 4331, Bangladesh.
Biomaterials Research Laboratory (BRL), Department of Applied Chemistry and Chemical Engineering, University of Chittagong, Chattogram, 4331, Bangladesh.
Heliyon. 2023 May 6;9(5):e16072. doi: 10.1016/j.heliyon.2023.e16072. eCollection 2023 May.
In this work, the aqueous leaf extracts of three genus species, namely (), () and (), have been used as the reducing and capping agents to control the size of AgNPs, -AgNPs, -AgNPs and -AgNPs, respectively and found to be an effective antimicrobial agent against a wide range of bacteria and fungi. The biosynthesized AgNPs were studied by UV-Visible spectrophotometer, powder X-ray diffraction (XRD), scanning electron microscopy (SEM), energy dispersive X-ray, transmission electron microscopy (TEM) and Fourier transform infrared spectrometer (FTIR). The average particle sizes of -AgNPs, -AgNPs and -AgNPs were measured as 17 nm, 22 nm and 26 nm, respectively, and observed to be spherical and face-centered cubic crystals. The antibacterial test of synthesized AgNPs was performed against , , , and where the maximum antibacterial activity was observed by reducing the nano-size and increasing the silver content of AgNPs. The antifungal effect of these three types of AgNPs on and was also evaluated and their growth with AgNPs concentrations of 450 μg/mL was inhibited up to 80-90% and 55-70%, respectively. The size-control synthesis of AgNPs using the genus species is presented here for the first time where the synthesized AgNPs showed higher stability and antimicrobial activities. Therefore, this study might lead to synthesize AgNPs with different morphologies using plant extracts of the same genus but from different species and provide strong encouragement for future applications in treating infectious diseases.
在本研究中，三种属植物物种，即（）、（）和（）的水提叶提取物分别被用作还原剂和封端剂来控制AgNPs、-AgNPs、-AgNPs和-AgNPs的尺寸，并且发现它们是针对多种细菌和真菌的有效抗菌剂。通过紫外可见分光光度计、粉末X射线衍射（XRD）、扫描电子显微镜（SEM）、能量色散X射线、透射电子显微镜（TEM）和傅里叶变换红外光谱仪（FTIR）对生物合成的AgNPs进行了研究。-AgNPs、-AgNPs和-AgNPs的平均粒径分别测定为17nm、22nm和26nm，并且观察到它们为球形和面心立方晶体。对合成的AgNPs针对、、、和进行了抗菌测试，其中通过减小纳米尺寸和增加AgNPs的银含量观察到了最大抗菌活性。还评估了这三种类型的AgNPs对和的抗真菌效果，并且在AgNPs浓度为450μg/mL时它们的生长分别被抑制高达80 - 90%和55 - 70%。首次在此展示了使用属植物物种进行AgNPs的尺寸控制合成，其中合成的AgNPs表现出更高的稳定性和抗菌活性。因此，本研究可能会导致使用同一属但不同物种的植物提取物合成具有不同形态的AgNPs，并为未来在治疗传染病中的应用提供有力支持。
