绿茶提取物表没食子儿茶素没食子酸酯（EGCG）对大鼠正畸牙齿移动和根吸收的影响。
Effects of green tea extract epigallocatechin-3-gallate (EGCG) on orthodontic tooth movement and root resorption in rats.
机构信息
Department of Orthodontics, School and Hospital of Stomatology, Shandong University & Shandong Provincial Key Laboratory of Oral Tissue Regeneration & Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration, Jinan, China.
Department of Stomatology, Rizhao Traditional Chinese Medicine Hospital, Rizhao, China.
出版信息
Arch Oral Biol. 2023 Jun;150:105691. doi: 10.1016/j.archoralbio.2023.105691. Epub 2023 Mar 29.
OBJECTIVE
To study the effect of EGCG on tooth movement and root resorption during orthodontic treatment in rats.
METHODS
A total of thirty six male Wistar rats were randomly and equally divided into three groups: control, 50 mg/kg EGCG, and 100 mg/kg EGCG. During the experiment, the subjects were submitted to an orthodontic tooth movement (OTM) model, rats in the experimental groups were given the corresponding dose of EGCG, while rats in the control group were administrated with an equal volume of normal saline solution by gavage. After 14 days of OTM, the rats were sacrificed by transcardial perfusion. Micro-CT of rat maxillaes was taken to analyze OTM distance and root resorption. The maxillary samples were prepared as histological sections for H&E staining, tartrate-resistant acid phosphatase (TRAP) staining and immunohistochemical (IHC) staining to be observed and analyzed.
RESULTS
The OTM distance and root resorption of rats in the dosed group decreased, and the number of TRAP positive cells in their periodontium decreased significantly. The expression level of RANKL was decreased in the EGCG group compared to the control group, while that of OPG, OCN and Runx2 was increased. Effects were more pronounced in 100 mg/kg group than in 50 mg/kg group.
CONCLUSION
EGCG reduces OTM and orthodontic induced root resorption (OIRR) in rats, and is able to attenuate osteoclastogenesis on the pressure side and promote osteogenesis on the tension side.
目的
研究 EGCG 对正畸治疗中牙齿移动和根吸收的影响。
方法
将 36 只雄性 Wistar 大鼠随机均分为三组：对照组、50mg/kg EGCG 组和 100mg/kg EGCG 组。实验过程中，所有大鼠均建立正畸牙齿移动（OTM）模型，实验组大鼠给予相应剂量的 EGCG，对照组大鼠给予等体积生理盐水灌胃。OTM 14 天后，通过心脏灌注处死大鼠。对大鼠上颌骨进行 micro-CT 扫描，分析 OTM 距离和根吸收情况。制备上颌组织学切片进行 H&E 染色、抗酒石酸酸性磷酸酶（TRAP）染色和免疫组化（IHC）染色，观察和分析。
结果
与对照组相比，给药组大鼠的 OTM 距离和根吸收减少，牙周组织中 TRAP 阳性细胞数量明显减少。与对照组相比，EGCG 组的 RANKL 表达水平降低，而 OPG、OCN 和 Runx2 的表达水平升高。100mg/kg 组的效果比 50mg/kg 组更显著。
结论
EGCG 可减少大鼠的 OTM 和正畸诱导的根吸收（OIRR），并能减弱压力侧的破骨细胞形成，促进张力侧的成骨作用。
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