Chemical Science Department (Biochemistry Unit), Joseph Ayo Babalola University, Ikeji-Arakeji, Nigeria.
Functional Foods and Nutraceutical Unit, Biochemistry Department, Federal University of Technology, Akure, Nigeria.
J Complement Integr Med. 2023 May 22;20(3):597-603. doi: 10.1515/jcim-2022-0390. eCollection 2023 Sep 1.
is one ethno-botanicals in Nigeria used by traditional health practitioners in managing several human diseases. However, necessary information regarding its effect on enzymes implicated in the development and progression of erectile dysfunction is missing in the literature. Thus, this study investigated the antioxidant property and impact of extract on erectile dysfunction-related enzymes
High performance liquid chromatography was used to identify and quantify 's phenolic components. Then, utilizing common antioxidant assays, the extract's antioxidant properties were evaluated and finally the effect of the extract on some enzymes (AChE, arginase and ACE) implicated in erectile dysfunction was investigated .
The results showed that the extract inhibited AChE (IC=388.72 μg/mL), arginase (IC=40.06 μg/mL) and ACE (IC=108.64 μg/mL) activities. In addition, phenolic rich extract of scavenged radicals and chelated Fe in concentration dependent manner. Furthermore, rutin, chlorogenic acid, gallic acid, and kaempferol were found in large quantities by HPLC analysis.
Therefore, one of the potential reasons driving 's use in folk medicine for the treatment of erectile dysfunction could be its antioxidant and inhibitory activities on several enzymes linked to erectile dysfunction .
是尼日利亚的一种民族植物药，传统的医疗从业者用它来治疗多种人类疾病。然而，关于它对与勃起功能障碍发展和进展相关的酶的影响的相关信息在文献中是缺失的。因此，本研究调查了 提取物的抗氧化特性及其对与勃起功能障碍相关的酶的影响。
采用高效液相色谱法鉴定和定量 的酚类成分。然后，利用常见的抗氧化测定法评估提取物的抗氧化特性，最后研究提取物对几种酶（AChE、精氨酸酶和 ACE）的影响，这些酶与勃起功能障碍有关。
结果表明，该提取物抑制 AChE（IC=388.72μg/mL）、精氨酸酶（IC=40.06μg/mL）和 ACE（IC=108.64μg/mL）的活性。此外，富含酚类的 提取物以浓度依赖的方式清除自由基和螯合 Fe。此外，通过 HPLC 分析发现大量的芦丁、绿原酸、没食子酸和山柰酚。
因此， 在民间医学中用于治疗勃起功能障碍的原因之一可能是其抗氧化和抑制与勃起功能障碍相关的几种酶的活性。
