Institute of Modern Biopharmaceuticals State Key Laboratory, Breeding Base Eco-Environment and Bio-Resource of the Three Gorges Area, Key Laboratory of Eco-environments in Three Gorges Reservoir Region, Ministry of Education, School of Life Sciences, Southwest University, Chongqing 400700, China.
Chongqing Public Health Medical Center, Southwest University Public Health Hospital, China.
Infect Genet Evol. 2023 Aug;112:105445. doi: 10.1016/j.meegid.2023.105445. Epub 2023 May 20.
CRISPR-Cas systems are the only RNA- guided adaptive immunity pathways that trigger the detection and destruction of invasive phages and plasmids in bacteria and archaea. Due to its prevalence and mystery, the Class 1 CRISPR-Cas system has lately been the subject of several studies. This review highlights the specificity of CRISPR-Cas system III-A in Mycobacterium tuberculosis, the tuberculosis-causing pathogen, for over twenty years. We discuss the difference between the several subtypes of Type III and their defence mechanisms. The anti-CRISPRs (Acrs) recently described, the critical role of Reverse transcriptase (RT) and housekeeping nuclease for type III CRISPR-Cas systems, and the use of this cutting-edge technology, its impact on the search for novel anti-tuberculosis drugs.
CRISPR-Cas 系统是唯一的 RNA 指导的适应性免疫途径，可触发细菌和古菌中入侵噬菌体和质粒的检测和破坏。由于其普遍性和神秘性，最近 Class 1 CRISPR-Cas 系统成为了多项研究的主题。本综述重点介绍了结核分枝杆菌（导致结核病的病原体）中 CRISPR-Cas 系统 III-A 二十多年来的特异性。我们讨论了几种 III 型亚型之间的差异及其防御机制。最近描述的抗 CRISPRs (Acrs)、逆转录酶 (RT) 和管家核酸酶对 III 型 CRISPR-Cas 系统的关键作用，以及这项尖端技术的应用，对寻找新型抗结核药物的影响。
