炎症细胞因子自身抗体作为阻塞性睡眠呼吸暂停患者的血清生物标志物

Autoantibodies of inflammatory cytokines as serum biomarkers in OSA patients.

作者信息

Fang Yifei, Su Jiao, Zhang Binglu, Zhao Chunling, Ji Longtao, Liang Feifei, Wang Zhi, Hao Jimin, Meng Yang, Wei Beilei, Huang Yuyang, Dai Liping, Ouyang Songyun

机构信息

Department of Respiratory and Sleep Medicine, the First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, Henan, China.

Henan Institute of Medical and Pharmaceutical Sciences & Henan Key Medical Laboratory of Tumor Molecular Biomarkers, Zhengzhou University, Zhengzhou 450052, Henan, China; BGI College, Zhengzhou University, Zhengzhou 450052, Henan, China.

出版信息

Clin Chim Acta. 2023 Jul 1;547:117399. doi: 10.1016/j.cca.2023.117399. Epub 2023 May 21.

DOI:10.1016/j.cca.2023.117399

PMID:37217113

Abstract

As many as 90% of patients with obstructive sleep apnea (OSA) may be undiagnosed. It is necessary to explore the potential value of autoantibodies against CRP, IL-6, IL-8 and TNF-α in the diagnosis of OSA. ELISA was performed to detect the level of autoantibodies against CRP, IL-6, IL-8 and TNF-α in sera from 264 OSA patients and 231 normal controls (NCs). The expression level of autoantibodies against CRP, IL-6 and IL-8 in OSA were significantly higher than that in NC while the level of anti-TNF-α was lower in OSA than that in NC. The per SD increment of anti-CRP, anti-IL-6 and anti-IL-8 autoantibodies were significantly associated with a 430%, 100% and 31% higher risk for OSA, respectively. The AUC of anti-CRP was 0.808 (95% CI: 0.771-0.845) when comparing OSA with NC, while the AUC increased to 0.876 (95% CI: 0.846-0.906) combining four autoantibodies. For discrimination of severe OSA versus NC and non-severe OSA versus NC, the AUC for four autoantibodies combination was 0.885 (95% CI: 0.851-0.918) and 0.876 (95% CI: 0.842-0.913). This study revealed the association between autoantibodies against inflammatory factors and OSA, and the combination of autoantibodies against CRP, IL-6, IL-8 and TNF-α may function as novel biomarker for monitoring the presence of OSA.

摘要

多达90%的阻塞性睡眠呼吸暂停（OSA）患者可能未被诊断出来。有必要探索抗CRP、IL-6、IL-8和TNF-α自身抗体在OSA诊断中的潜在价值。采用酶联免疫吸附测定（ELISA）检测264例OSA患者和231例正常对照（NC）血清中抗CRP、IL-6、IL-8和TNF-α自身抗体水平。OSA患者中抗CRP、IL-6和IL-8自身抗体的表达水平显著高于NC，而OSA患者中抗TNF-α水平低于NC。抗CRP、抗IL-6和抗IL-8自身抗体每增加1个标准差，OSA风险分别显著增加430%、100%和31%。将OSA与NC进行比较时，抗CRP的曲线下面积（AUC）为0.808（95%可信区间：0.771-0.845），而将四种自身抗体联合检测时，AUC增至0.876（95%可信区间：0.846-0.906）。对于区分重度OSA与NC以及非重度OSA与NC，四种自身抗体联合检测的AUC分别为0.885（95%可信区间：0.851-0.918）和0.876（95%可信区间：0.842-0.913）。本研究揭示了抗炎症因子自身抗体与OSA之间的关联，抗CRP、IL-6、IL-8和TNF-α自身抗体的联合检测可能作为监测OSA存在的新型生物标志物。