School of Chemistry and Chemical Engineering, Wuhan University of Science and Technology, 947 Heping Road, Wuhan 430081, People's Republic of China.
School of Chemistry and Chemical Engineering, Wuhan University of Science and Technology, 947 Heping Road, Wuhan 430081, People's Republic of China; HuBei Province Key Laboratory of Coal Conversion and New Carbon Materials, Wuhan University of Science and Technology, Wuhan 430081, People's Republic of China.
Bioresour Technol. 2023 Aug;382:129218. doi: 10.1016/j.biortech.2023.129218. Epub 2023 May 20.
Pretreatment is a prerequisite to tackle the issue of biomass recalcitrance, which is the major hindrance of lignocellulose-to-sugars routes. In the present study, a novel combination of dilute sulfuric acid (dilute-HSO) with Tween 80 pretreatment of corn stover (CS) was developed to significantly enhance the enzyme digestibility. Strong synergistic effects of HSO and Tween 80 for simultaneously eliminating hemicellulose and lignin and significantly promoting saccharification yield were observed. A response surface optimization realized the maximum monomeric sugar yield of 95.06% at 120 °C for 1.4 h with 0.75 wt% of HSO and 73.92 wt% of Tween 80. The excellent enzyme susceptibility of pretreated CS was explained by their physical and chemical characteristics via SEM, XRD, and FITR. The repeatedly recovered pretreatment liquor exerted highly-effective reusability in the subsequent pretreatments for at least four cycles. This strategy offers a highly-efficient and practical pretreatment strategy, which provides valuable information for the lignocellulose-to-sugars routes.
预处理是解决生物质顽固性问题的前提，这是木质纤维素转化为糖的主要障碍。在本研究中，开发了一种新型的稀硫酸（dilute-HSO）与吐温 80 联合预处理玉米秸秆（CS）的方法，可显著提高酶解效率。观察到 HSO 和吐温 80 之间具有很强的协同作用，可同时去除半纤维素和木质素，并显著提高糖化产率。响应面优化实现了在 120°C 下反应 1.4 小时、HSO 用量为 0.75wt%、吐温 80 用量为 73.92wt%时，单体糖得率最高可达 95.06%。通过 SEM、XRD 和 FTIR 对预处理 CS 的物理化学特性进行了解释，发现其具有良好的酶解性能。重复回收的预处理液在随后的预处理中至少可重复使用 4 次，具有很高的有效性和实用性。该策略提供了一种高效实用的预处理策略，为木质纤维素转化为糖的途径提供了有价值的信息。
