Encapsulating functionalized graphene quantum dot into metal-organic framework as a ratiometric fluorescent nanoprobe for doxycycline sensing.

A distinctive fluorescent nanoprobe with the function of doxycycline identification was designed by encapsulating histidine and serine-functionalized graphene quantum dots (His-GQDs-Ser) into the luminescent metal-organic frameworks (MOF). The synthesized nanoprobe displayed the merits of prominent selectivity, wide detection range, and high sensitivity. The interaction of doxycycline and the fabricated fluorescent nanoprobe contributed to the phenomenon of the suppression of the fluorescence of the His-GQDs-Ser and enhancement of the MOF fluorescence. Linear relation between the concentration of doxycycline and the ratio fluorescence intensity of the nanoprobe was observed, which evidenced the brilliant capability in the ranges 0.003-6.25 μM and 6.25-25 μM with a detection limit of 1.8 nM. Additionally, the practicability of the probe was verified in analysis of spiked milk sample, and the satisfactory recoveries of doxycycline varied from 97.39 to 103.61%, with relative standard deviations in the range 0.62-1.42%. A proportional fluorescence sensor for doxycycline detection in standard solution was constructed, which provides a potential for the development of other fluorescence detection systems.