Steinbeck R, Steinbeck C, Postel E, Busse H, Havsteen B, Auer G
Histochemistry. 1986;84(4-6):338-41. doi: 10.1007/BF00482960.
Two-dimensional-electrophoretic analysis has been applied to non-neoplastic mammary epithelium from eight healthy women, tumor tissue from eight "diploid" mammary adenocarcinomas, and tumor tissue from eight "aneuploid" mammary adenocarcinomas. Compared with non-neoplastic mammary epithelium, a slight numerical net non-neoplastic mammary epithelium, a slight numerical net increase of the protein spots was detected in "diploid" tumors and a marked increase in "aneuploid" tumors. Two prominent spots were present in all 16 malignant tissues examined and absent in all eight non-neoplastic tissues (silver staining method). The results suggest that a difference in the composition of cellular proteins exists both between non-neoplastic mammary cells and malignant tumor cells, and between "diploid" and "aneuploid" tumors.
二维电泳分析已应用于8名健康女性的非肿瘤性乳腺上皮、8例“二倍体”乳腺腺癌的肿瘤组织以及8例“非整倍体”乳腺腺癌的肿瘤组织。与非肿瘤性乳腺上皮相比，在“二倍体”肿瘤中检测到蛋白质斑点数量略有净增加，而在“非整倍体”肿瘤中则显著增加。在所有检测的16个恶性组织中均存在两个突出的斑点，而在所有8个非肿瘤组织中均未出现（银染法）。结果表明，非肿瘤性乳腺细胞与恶性肿瘤细胞之间以及“二倍体”和“非整倍体”肿瘤之间存在细胞蛋白质组成的差异。
