Neerunjun J S, Dubowitz V
J Neurol Sci. 1977 Aug;33(1-2):95-109. doi: 10.1016/0022-510x(77)90185-x.
Regenerating fibres from tibialis anterior muscles of mice and hamsters transplanted as minced fragments for 7 and 9 days respectively were compared for basophilia, ribonucleic acid (RNA) and acid phosphatase activity with fibres in muscles of patients with Duchenne muscular dystrophy, limb--girdle dystrophy and dermatomyositis. Normal muscles of mice, hamsters and humans were used as controls. In normal muscle fibres basophilia and RNA activity were restricted to the nuclei and acid phosphatase activity was occasionally observed in the nuclei, endomysial connective tissue and around muscle spindles. Diseased human muscle fibres were rich in acid phosphatase activity, the enzyme being most prominent in degenerating and basophilic fibres. When examined in serial sections, all basophilic fibres showed RNA and acid phosphatase activity. Similarly, all regenerating fibres in minced transplants which were basophilic to haematoxylin contained high RNA and acid phosphatase activity. It is suggested that basophilia in diseased human muscle fibres represents regeneration and that the lysosomes, as defined by acid phosphatase activity in these fibres, may be promoting their growth and differentiation by facilitating greater nucleocytoplasmic communication which ultimately could lead to protein synthesis.
将分别移植了7天和9天的小鼠和仓鼠胫骨前肌切碎片段再生的纤维，与杜氏肌营养不良症、肢带型肌营养不良症和皮肌炎患者肌肉中的纤维进行嗜碱性、核糖核酸（RNA）和酸性磷酸酶活性比较。以小鼠、仓鼠和人类的正常肌肉作为对照。在正常肌纤维中，嗜碱性和RNA活性局限于细胞核，酸性磷酸酶活性偶尔见于细胞核、肌内膜结缔组织和肌梭周围。患病的人类肌纤维富含酸性磷酸酶活性，该酶在退化和嗜碱性纤维中最为显著。在连续切片检查时，所有嗜碱性纤维均显示RNA和酸性磷酸酶活性。同样，在切碎移植中所有对苏木精呈嗜碱性的再生纤维都含有高RNA和酸性磷酸酶活性。有人提出，患病人类肌纤维中的嗜碱性代表再生，并且这些纤维中由酸性磷酸酶活性所界定的溶酶体，可能通过促进更大的核质通讯来促进其生长和分化，而这最终可能导致蛋白质合成。
