Beckman W C, Fried F A, Stafford S J, Mickey D D
J Androl. 1986 May-Jun;7(3):197-202. doi: 10.1002/j.1939-4640.1986.tb00911.x.
The human vas deferens was examined autoradiographically for the presence and distribution of androgen receptors. Samples of vas deferens from the region proximal to the testis and the region at the internal inguinal ring were incubated in vitro with tritium-labeled dihydrotestosterone ([3H]-DHT). Frozen sections of tissue were mounted on autoradiographic emulsion-coated slides and exposed for up to three weeks to demonstrate cells with nuclear accumulations of radioactive hormone. Quantitation of autoradiograms was performed with a Zeiss Videoplan morphometric analysis system. Cells in all five tissue layers of the vas deferens were able to bind androgen receptors in the nucleus, as evidenced by superimposition of silver grains over the nuclei of cells in external, middle, and internal smooth muscle layers, as well as in epithelial and subepithelial stromal cells.
对人类输精管进行放射自显影检查，以确定雄激素受体的存在和分布。将来自睾丸近端区域和腹股沟内环区域的输精管样本与氚标记的双氢睾酮（[3H]-DHT）进行体外孵育。将组织冰冻切片安装在涂有放射自显影乳剂的载玻片上，并曝光长达三周，以显示细胞核内有放射性激素聚集的细胞。使用蔡司图像分析系统对放射自显影片进行定量分析。输精管所有五层组织中的细胞都能够在细胞核中结合雄激素受体，外部、中间和内部平滑肌层以及上皮和上皮下基质细胞的细胞核上银粒的叠加证明了这一点。
