Department of Pharmaceutics and Biopharmaceutics, University of Marburg, Robert-Koch-Str. 4, 35037 Marburg, Germany.
Center for Tumor Biology and Immunology (ZTI), Core Facility Molecular Imaging, Department of Nuclear Medicine, University of Marburg, Hans-Meerwein-Str. 3, 35043 Marburg, Germany.
ACS Appl Bio Mater. 2023 Jun 19;6(6):2435-2445. doi: 10.1021/acsabm.3c00237. Epub 2023 May 24.
The chorioallantoic membrane (CAM) of fertilized hen's eggs represents a unique and alternative model for cancer research. The CAM model provides an optimal platform for xenografting cancer cell lines and studying essential key factors. Tumor size and growth as well as angiogenesis can be investigated to evaluate the response of therapies and strategies against cancer. Preclinical imaging represented by magnetic resonance imaging and positron emission tomography/computed tomography can generate detailed anatomical and functional information and reveal excellent metabolic sensitivity. In the following, a guideline is introduced in order to find a simplified entrance to the CAM model in combination with modern preclinical imaging techniques. Finally, the presented procedures are additionally completed by histological studies in the form of hematoxylin and eosin as well as immunohistochemical staining.
鸡胚的尿囊膜（CAM）是一种独特的、可替代的癌症研究模型。CAM 模型为异种移植癌细胞系和研究基本关键因素提供了最佳平台。肿瘤的大小和生长以及血管生成可以被研究，以评估治疗和抗癌策略的反应。以磁共振成像和正电子发射断层扫描/计算机断层扫描为代表的临床前成像可以生成详细的解剖学和功能信息，并显示出极好的代谢敏感性。在下面，引入了一个指南，以便结合现代临床前成像技术找到 CAM 模型的简化入口。最后，通过苏木精和伊红以及免疫组织化学染色的组织学研究来补充所提出的程序。
