Laboratory Department, Luxor Fever and GIT Hospital, Luxor, Egypt.
Department of Biotechnology and Life Sciences, Faculty of Postgraduate Studies for Advanced Sciences, Beni-Suef University, Beni-Suef, Egypt.
Immunogenetics. 2023 Aug;75(4):403-410. doi: 10.1007/s00251-023-01308-6. Epub 2023 May 24.
Following the announcement of the pandemic of COVID-19 in December 2019, several studies focused on how to early predict the severity of the disease in symptomatic and asymptomatic patients. Many cytokines including interleukin-6, interleukin-8, and tumor necrotic factors have been concluded as strong indicators for COVID-19 infection. Additionally, miRNAs have been associated with dysregulation in the immune system. The aim of this study are the following: (1) to estimate the level of miRNA-16-2-3P, miRNA-618, IL-8, IL-1β as predictors for SARS-CoV-2 complications in PCR negative and positive patients; (2) to assess the biological role and effect of these miRNAs on SARS-CoV-2 pathogenicity. Our study showed that the level of IL-1β had been significantly associated with patient who need hospitalization, also the alteration of the level of miRNA-16-2-3P, miRNA-618 is positively correlated with the admission of these patients and influence the outcomes of SARS-cov-2 infection. Measurement of miRNA-16-2-3P, miRNA-618, IL-1β could be a good predictor of COVID-19 patient outcome. However the measurement of IL-8 levels during immune responses in the admitted and in ICU patients could have a prognostic value.
继 2019 年 12 月宣布 COVID-19 大流行以来，多项研究集中于如何早期预测有症状和无症状患者疾病的严重程度。许多细胞因子，包括白细胞介素-6、白细胞介素-8 和肿瘤坏死因子，已被证明是 COVID-19 感染的强有力指标。此外，miRNA 与免疫系统失调有关。本研究的目的如下：（1）评估 miRNA-16-2-3P、miRNA-618、IL-8、IL-1β 在 PCR 阴性和阳性患者中作为 SARS-CoV-2 并发症预测因子的水平；（2）评估这些 miRNA 对 SARS-CoV-2 致病性的生物学作用和影响。我们的研究表明，IL-1β 的水平与需要住院的患者显著相关，miRNA-16-2-3P、miRNA-618 水平的改变与这些患者的入院呈正相关，并影响 SARS-cov-2 感染的结果。miRNA-16-2-3P、miRNA-618、IL-1β 的测量可能是 COVID-19 患者预后的良好预测因子。然而，在住院和 ICU 患者的免疫反应期间测量 IL-8 水平可能具有预后价值。
