Department of Pathology, HUSLAB, Helsinki University Hospital and University of Helsinki, FIN-00290, Helsinki, Finland.
University of Helsinki, FIN-00290, Helsinki, Finland.
Virchows Arch. 2023 Aug;483(2):215-224. doi: 10.1007/s00428-023-03563-0. Epub 2023 May 24.
Core needle biopsies (CNB) are widely used to diagnose breast cancer, but the procedure is invasive and thus, it changes the tumor microenvironment. The purpose of this study is to see how the expression of three potentially anti-inflammatory molecules, namely, programmed death-ligand 1 (PD-L1), sialic acid-binding immunoglobulin-like lectin-15 (Siglec-15), and C-C chemokine receptor-5 (CCR-5), are expressed in CNB and surgical resection specimens (SRS). To do this, we compared the amounts of tumor-infiltrating lymphocytes and the levels of CCR5, Siglec-15, and PD-L1 in tumor cells and inflammatory cells as assessed by immunohistochemistry in CNB and the corresponding SRS of 22 invasive breast carcinomas of no special type and 22 invasive lobular carcinomas. The Siglec-15 H-score was higher in tumor cells in the SRS than in the CNB groups. There was no change in tumor cells CCR5 or PD-L1 between CNB and SRS. The positive inflammatory cell numbers for all markers rose between CNB and SRS, as did the amount of Tils. Furthermore, higher grade tumors and tumors with a high proliferation rate had more inflammatory cells that were positive for the markers and also more PD-L1+ tumor cells. Although changes in inflammatory cells can partly be attributed to the larger sample size of operation specimens, the differences also mirror a true change in the tumor microenvironment. The changes in inflammatory cells could be partly due to the need to restrict excess inflammation at the site of the biopsy.
核心针活检（CNB）广泛用于诊断乳腺癌，但该过程具有侵入性，因此会改变肿瘤微环境。本研究的目的是观察三种潜在抗炎分子（程序性死亡配体 1（PD-L1）、唾液酸结合免疫球蛋白样凝集素 15（Siglec-15）和 C-C 趋化因子受体 5（CCR-5））在 CNB 和手术切除标本（SRS）中的表达情况。为此，我们比较了 22 例非特殊类型浸润性乳腺癌和 22 例浸润性小叶癌的 CNB 和相应的 SRS 中肿瘤浸润淋巴细胞的数量以及通过免疫组织化学评估的肿瘤细胞和炎症细胞中 CCR5、Siglec-15 和 PD-L1 的水平。Siglec-15 H 评分在 SRS 中的肿瘤细胞中高于 CNB 组。肿瘤细胞的 CCR5 或 PD-L1 在 CNB 和 SRS 之间没有变化。所有标志物的阳性炎症细胞数量在 CNB 和 SRS 之间均增加，Tils 数量也增加。此外，较高等级的肿瘤和具有高增殖率的肿瘤具有更多的炎症细胞阳性标志物和更多的 PD-L1+肿瘤细胞。尽管炎症细胞的变化部分归因于手术标本的更大样本量，但这些差异也反映了肿瘤微环境的真实变化。炎症细胞的变化可能部分是由于需要限制活检部位的过度炎症。
