生物素偶联抗血清在植物病毒免疫测定中的应用。

The use of biotin-conjugated antisera in immunoassays for plant viruses.

作者信息

Hewish D R, Shukla D D, Gough K H

出版信息

J Virol Methods. 1986 Apr;13(1):79-85. doi: 10.1016/0166-0934(86)90075-3.

Abstract

Biotin-conjugated antisera to two strains of sugarcane mosaic virus and erysimum latent virus were used to detect the viruses in extracts of infected plants. Two methods, enzyme-linked immunosorbent assays and electroblot immunoassays, were used. The antisera were found to be sufficiently sensitive for detection of the viruses. Virus strain specificities observed for the antisera agreed with those found using immunoelectron microscopy and electroblot immunoassay. The sensitivity of biotin conjugated antiserum was compared with that of peroxidase conjugated antiserum. It was found that biotin conjugation gave increased sensitivity. The Biotin-avidin system offers several advantages over other current methods of antibody labelling, notably in speed of development and versatility.

摘要

用生物素偶联的抗两种甘蔗花叶病毒和糖芥潜隐病毒毒株的抗血清检测感病植株提取物中的病毒。采用了两种方法，即酶联免疫吸附测定法和电印迹免疫测定法。结果发现这些抗血清对病毒的检测灵敏度足够高。观察到的抗血清的病毒毒株特异性与使用免疫电子显微镜和电印迹免疫测定法所发现的一致。将生物素偶联抗血清的灵敏度与过氧化物酶偶联抗血清的灵敏度进行了比较。结果发现生物素偶联提高了灵敏度。生物素-抗生物素蛋白系统相对于目前其他抗体标记方法具有若干优势，尤其是在显影速度和通用性方面。