Shukla D D, McKern N M, Ward C W
CSIRO, Division of Biotechnology, Parkville Laboratory, Victoria, Australia.
Arch Virol. 1988;102(3-4):221-32. doi: 10.1007/BF01310827.
Three strains of passionfruit woodiness virus, Tip Blight (PWV-TB), Severe (PWV-S) and Mild (PWV-M), were compared on the basis of their biological, serological and coat protein structural properties. Each of the strains could be distinguished on the basis of their reactions on selected test plant species but no differences were observed in the serological properties of the three PWV strains. Molecular weight estimates on SDS-PAGE suggest the PWV coat protein contains 275 amino acid residues and sequence data for 269 of these residues is presented. The amino terminal peptide is blocked and has not been sequenced. The coat proteins of PWV-TB and PWV-S, which cause severe symptoms in infected plants, showed only three sequence differences compared to the eleven or twelve sequence changes between their coat proteins and that of the mild strain. The high sequence homology (96-99%) between the three PWV strains is similar to that previously reported for two strains of tobacco etch virus and three strains of sugarcane mosaic virus. Sequence homology between the three strains of PWV and published sequences for the coat proteins of eight distinct potyviruses ranged from 43-71% (average 57%).
对百香果木质化病毒的三个株系，即梢枯株系（PWV-TB）、严重株系（PWV-S）和温和株系（PWV-M），基于其生物学、血清学和外壳蛋白结构特性进行了比较。每个株系可根据它们在选定试验植物物种上的反应加以区分，但未观察到这三个百香果木质化病毒株系在血清学特性上存在差异。SDS-PAGE上的分子量估计表明，百香果木质化病毒外壳蛋白含有275个氨基酸残基，并给出了其中269个残基的序列数据。氨基末端肽被封闭，尚未测序。在受感染植物中引起严重症状的PWV-TB和PWV-S的外壳蛋白，与温和株系的外壳蛋白相比，仅显示出三个序列差异，而它们自身之间的外壳蛋白则有11个或12个序列变化。这三个百香果木质化病毒株系之间的高序列同源性（96 - 99%），与之前报道的烟草蚀纹病毒两个株系和甘蔗花叶病毒三个株系的情况相似。这三个百香果木质化病毒株系与已发表的八个不同马铃薯Y病毒外壳蛋白序列之间的同源性在43 - 71%（平均57%）之间。
