Milewich L, MacDonald P C, Carr B R
J Clin Endocrinol Metab. 1986 Aug;63(2):404-6. doi: 10.1210/jcem-63-2-404.
Estrogen 16 alpha-hydroxylase activity was measured in microsomes prepared from fetal tissues of first and second trimester human abortuses using [16 alpha-3H]estrone sulfate as substrate and NADPH as cofactor. Estrogen 16 alpha-hydroxylase activity was demonstrable in 13 of 14 fetal tissues examined in this study, viz. liver, adrenal fetal zone, adrenal neocortex, lung, kidney, intestine, heart, brain, skin, testis, spleen, pancreas, and stomach, and was either negligible or absent in placental tissue. The highest specific activity of the microsomal enzyme [pico-moles of product(s) formed per mg protein/h] was found in liver (mean +/- SEM, 338 +/- 62), and the next highest was found in the fetal zone of the adrenal cortex (70 +/- 20). The specific activities of estrogen 16 alpha-hydroxylase in adrenal neocortex, brain, skin, and testis were similar (25-53 pmol/mg protein X h) as were those in lung, kidney, intestine, heart, spleen and stomach (23-36 pmol/mg protein X h). The specific activity of the enzyme in the pancreas was 12 pmol/mg protein X h; the lowest specific activity, however, was in placental microsomes (0.2 +/- 0.1 pmol/mg protein X h).
以[16α-³H]硫酸雌酮为底物、NADPH为辅助因子，检测了取自妊娠早期和中期人工流产胎儿组织制备的微粒体中的雌激素16α-羟化酶活性。本研究检测的14种胎儿组织中有13种检测到雌激素16α-羟化酶活性，即肝脏、肾上腺胎儿带、肾上腺新皮质、肺、肾、肠、心脏、脑、皮肤、睾丸、脾脏、胰腺和胃，而胎盘组织中的该酶活性可忽略不计或不存在。微粒体酶的最高比活性[每毫克蛋白质每小时形成的产物皮摩尔数]见于肝脏（平均值±标准误，338±62），其次是肾上腺皮质胎儿带（70±20）。肾上腺新皮质、脑、皮肤和睾丸中雌激素16α-羟化酶的比活性相似（25 - 53皮摩尔/毫克蛋白质×小时），肺、肾、肠、心脏、脾脏和胃中的比活性也相似（23 - 36皮摩尔/毫克蛋白质×小时）。胰腺中该酶的比活性为12皮摩尔/毫克蛋白质×小时；然而，胎盘微粒体中的比活性最低（0.2±0.1皮摩尔/毫克蛋白质×小时）。
