Cappuccio F P, Sagnella G A, Leathard H L, Markandu N D, MacGregor G A
J Clin Endocrinol Metab. 1986 Aug;63(2):463-7. doi: 10.1210/jcem-63-2-463.
We studied the effect of plasma from 12 patients with essential hypertension and 12 normotensive subjects on the contractile response to norepinephrine in human isolated arterial spiral strips. Human mesenteric and uterine arteries were obtained during abdominal surgery; they were cut into spiral strips and set up in isolated organ baths. After the equilibration period, arterial strips were incubated for 20 min in plasma from either normotensive subjects or hypertensive patients, and the contractile responses to norepinephrine (2.96 X 10(-7) M) were recorded. Plasma from hypertensive subjects significantly increased the contractile response to norepinephrine by 25.8% (P less than 0.02). Plasma from normotensive subjects did not increase the contractile response to the pressor agent (-3.2%; P = NS). The mean change in contractile response to norepinephrine in the presence of plasma from hypertensive patients was significantly higher than that after incubation of the human arterial strips in plasma from normotensive subjects (P less than 0.02). When both groups were considered as a whole, there was a significant correlation between diastolic pressure and the change in the contractile response to norepinephrine (r = 0.52; P less than 0.01). These results suggest the existence of a circulating vascular sensitizing substance in patients with essential hypertension.
我们研究了12例原发性高血压患者和12例血压正常者的血浆对人离体动脉螺旋条去甲肾上腺素收缩反应的影响。人肠系膜动脉和子宫动脉取自腹部手术；将其切成螺旋条并置于离体器官浴槽中。平衡期后，动脉条在血压正常者或高血压患者的血浆中孵育20分钟，并记录对去甲肾上腺素（2.96×10⁻⁷M）的收缩反应。高血压患者的血浆使对去甲肾上腺素的收缩反应显著增加了25.8%（P＜0.02）。血压正常者的血浆未增加对升压药的收缩反应（-3.2%；P=无统计学意义）。在存在高血压患者血浆的情况下，对去甲肾上腺素收缩反应的平均变化显著高于人动脉条在血压正常者血浆中孵育后的变化（P＜0.02）。将两组作为一个整体考虑时，舒张压与对去甲肾上腺素收缩反应的变化之间存在显著相关性（r=0.52；P＜0.01）。这些结果提示原发性高血压患者存在一种循环血管致敏物质。
