Department of Clinical Laboratory, Nanjing Gaochun People's Hospital, Nanjing, Jiangsu, 211300, China.
College of Life Science and Technology, Huazhong Agricultural University, Wuhan, Hubei, 430070, China.
Cell Mol Biol (Noisy-le-grand). 2023 Feb 28;69(2):121-125. doi: 10.14715/cmb/2023.69.2.20.
To study the expressions of follicular helper T cell (Tfh) subsets--Tfh1, Tfh2, and Tfh17, in peripheral blood (PB) of primary Sjogren's syndrome (PSS) patients in the active phase and remission period after treatment, and to analyze the pathogenic effects of Tfh subsets in PSS patients. The proportions of Tfh1, Tfh2, and Tfh17 were measured by flow cytometry in the healthy group, PSS patients group, active phase group, and remission phase group. Enzyme-linked immunosorbent assay was used to detect IL-21 expression in SS patients in active and remission stages. Biomedical statistics was used to analyze the correlation between Tfh subsets and SS disease activity index; and analyze the correlation between the proportions of Tfh subsets in the healthy group, primary group, active stage, and remission stage In contrast with the healthy group, the levels of Tfh1, Tfh2, and Tfh17 in CD4+ T cells of PSS patients were prominently reduced. PSS patients in the active phase had significantly lower Tfh1, Tfh2, and Tfh17 levels but remarkably higher IL-21 levels than in the remission phase. The contents of Tfh1, Tfh2, and Tfh17 are negatively correlated with the severity of PSS.
研究原发性干燥综合征（PSS）患者活动期和治疗缓解期外周血滤泡辅助 T 细胞（Tfh）亚群（Tfh1、Tfh2 和 Tfh17）的表达，并分析 Tfh 亚群在 PSS 患者中的致病作用。采用流式细胞术检测健康组、PSS 患者组、活动期组和缓解期组 Tfh1、Tfh2 和 Tfh17 的比例。采用酶联免疫吸附试验检测 SS 患者活动期和缓解期的 IL-21 表达。采用生物医学统计学方法分析 Tfh 亚群与 SS 疾病活动指数的相关性；分析健康组、原发性组、活动期组和缓解期组 Tfh 亚群比例的相关性与健康组相比，PSS 患者 CD4+T 细胞中 Tfh1、Tfh2 和 Tfh17 的水平明显降低。活动期 PSS 患者 Tfh1、Tfh2 和 Tfh17 水平明显降低，但 IL-21 水平明显升高，缓解期。Tfh1、Tfh2 和 Tfh17 的含量与 PSS 的严重程度呈负相关。
