Department of Biology, College of Science and Arts, Al-Baha University, Baljurashi, Saudi Arabia.
Cell Mol Biol (Noisy-le-grand). 2023 Feb 28;69(2):126-132. doi: 10.14715/cmb/2023.69.2.21.
There is great plant diversity in Saudi Arabia. The Asphodelaceae family is within this great diversity, especially the rare species such as the plant, Aloe saudiarabica. Such plants must be preserved in their natural ranges, hence, the need to document them. Genetic markers have become the approved and widely used method for documenting rare plants. The current study deals with the use of three genetic markers to document A. saudiarabica for the first time. The used genetic markers were Maturase-K (matK), Ribulose-bisphosphate-carboxylase (rbcL), and Internal-transcribed-spacer (ITS). The study found that the primers used for the rbcL gene were not effective in achieving identification. Sequencing of the matK and ITS were achieved successfully. The sequences were determined for both markers using two pairs of primers and deposited in the NCBI databases (GenBank). These markers were effective in identifying A. saudiarabica and determining its evolutionary relationship with other Aloe species in various databases. The study showed that A. vera is high similar (>99%) to the other species. In conclusion, the study showed the likelihood of the different genetic markers to document A. saudiarabica, especially the currently investigated matK and ITS.
沙特阿拉伯拥有丰富的植物多样性。天门冬科家族就是这种多样性的一部分，特别是一些珍稀物种，如植物，阿拉伯芦荟。这些植物必须在其自然范围内得到保护，因此需要对它们进行记录。遗传标记已成为记录珍稀植物的公认和广泛使用的方法。本研究首次使用三种遗传标记来记录 A. saudiarabica。所使用的遗传标记是 maturase-K (matK)、核酮糖二磷酸羧化酶 (rbcL) 和内转录间隔区 (ITS)。研究发现，用于 rbcL 基因的引物在鉴定方面效果不佳。成功地对 matK 和 ITS 进行了测序。使用两对引物对这两个标记进行了序列测定，并将其保存在 NCBI 数据库 (GenBank) 中。这些标记可有效识别 A. saudiarabica，并确定其与各种数据库中其他芦荟物种的进化关系。研究表明，A. vera 与其他物种高度相似（>99%）。总之，该研究表明，不同的遗传标记有可能记录 A. saudiarabica，特别是目前研究的 matK 和 ITS。
