通过定性和定量激光烧蚀生物成像研究银纳米粒子对大豆种植中金属内稳性的作用。
The role of silver nanoparticles effects in the homeostasis of metals in soybean cultivation through qualitative and quantitative laser ablation bioimaging.
机构信息
Spectrometry, Sample Preparation and Mechanization Group, Institute of Chemistry, University of Campinas, Unicamp, P.O. Box 6154, Campinas, SP 13083-970, Brazil; National Institute of Science and Technology for Bioanalytics, Institute of Chemistry, University of Campinas, Unicamp, P.O. Box 6154, Campinas, SP 13083-970, Brazil.
Spectrometry, Sample Preparation and Mechanization Group, Institute of Chemistry, University of Campinas, Unicamp, P.O. Box 6154, Campinas, SP 13083-970, Brazil; National Institute of Science and Technology for Bioanalytics, Institute of Chemistry, University of Campinas, Unicamp, P.O. Box 6154, Campinas, SP 13083-970, Brazil.
出版信息
J Trace Elem Med Biol. 2023 Sep;79:127207. doi: 10.1016/j.jtemb.2023.127207. Epub 2023 May 18.
BACKGROUND
Nanoparticles (NPs) are currently found in the world in the form of natural colloids and volcanic ash, as well as in anthropogenic sources, such as nanofertilizers; however, in the literature, there is still a lack of toxicological evidence, risk assessment, and regulations about the use and environmental impact of NPs in the agroindustrial system. Therefore, the aim of this work was to evaluate alterations caused by the presence of AgNPs during the development of soybean plants.
METHODS
The BRS232 non-transgenic (NT) soybean plant and 8473RR (T) and INTACTA RR2 PRO (T) transgenic soybean plants were irrigated for 18 days under controlled conditions with deionized water (control), AgNPs, and AgNO. The isotopes Ag, Mn, Fe, Cu, and Zn were mapped in leaves, using C as an internal standard (IS), and carried out using a laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) technique with a Nd:YAG (213 nm) laser source in the imagagin mode using the LA-iMageS software and also Mathlab.
RESULTS
Leaf images showed a low Ag translocation, indicated by the basal signal of this ion. Additionally, the presence of Ag in the ionic form and as NPs altered the homeostasis of Cd, Zn, Mn, Cu, and Fe in different ways. Quantitative image analysis was performed for Cu.
CONCLUSION
The behavior of T and T plants was different in the presence of ionic silver or AgNPs, confirming that the metabolism of these two plants, despite both being transgenic, are different. Through the images, it was observed that the response of plants was different in the face of the same stress conditions during their development.
背景
纳米颗粒（NPs）目前以天然胶体和火山灰的形式存在于自然界中，以及人为来源，如纳米肥料；然而，在文献中，仍然缺乏关于 NPs 在农业系统中的使用和环境影响的毒理学证据、风险评估和法规。因此，本工作的目的是评估 AgNPs 的存在对大豆植株发育过程中造成的影响。
方法
在受控条件下，用去离子水（对照）、AgNPs 和 AgNO 对 BRS232 非转基因（NT）大豆植株和 8473RR（T）和 INTACTA RR2 PRO（T）转基因大豆植株进行 18 天的灌溉。使用激光烧蚀电感耦合等离子体质谱（LA-ICP-MS）技术，以 Nd:YAG（213nm）激光源在成像模式下，以 C 作为内标（IS），对叶片中的 Ag、Mn、Fe、Cu 和 Zn 进行了定位，并使用 LA-iMageS 软件和 Mathlab 进行了分析。
结果
叶片图像显示 Ag 的转运量较低，这表明该离子的基信号较低。此外，离子态银和 NPs 的存在以不同的方式改变了 Cd、Zn、Mn、Cu 和 Fe 的体内平衡。对 Cu 进行了定量图像分析。
结论
在存在离子银或 AgNPs 的情况下，T 和 T 植物的行为不同，这证实了尽管这两种植物都是转基因的，但它们的代谢是不同的。通过图像观察到，在植物发育过程中面临相同的胁迫条件时，它们的反应是不同的。
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